Structural insights into chromosome attachment to the nuclear envelope by an inner nuclear membrane protein Bqt4 in fission yeast

doi:10.1093/nar/gky1186

	Structural insights into chromosome attachment to the nuclear envelope by an inner nuclear membrane protein Bqt4 in fission yeast
	Hu, Chunyi 1,2; Inoue, Haruna 3; Sun, Wenqi4 ; Takeshita, Yumiko 3; Huang, Yaoguang 1,2; Xu, Ying 1,2; Kanoh, Junko 3; Chen, Yong1,2,4
	2019-02-20
发表期刊	NUCLEIC ACIDS RESEARCH (IF:16.6[JCR-2023],16.1[5-Year])
ISSN	0305-1048
卷号	47 期号:3 页码:1573-1584
发表状态	已发表
DOI	10.1093/nar/gky1186
摘要	The dynamic association of chromosomes with the nuclear envelope (NE) is essential for chromosome maintenance. Schizosaccharomyces pombe inner nuclear membrane protein Bqt4 plays a critical role in connecting telomeres to the NE, mainly through a direct interaction with the telomeric protein Rap1. Bqt4 also interacts with Lem2 for pericentric heterochromatin maintenance. How Bqt4 coordinates the interactions with different proteins to exert their functions is unclear. Here, we report the crystal structures of the N-terminal domain of Bqt4 in complexes with Bqt4-binding motifs from Rap1, Lem2, and Sad1. The structural, biochemical and cellular analyses reveal that the N-terminal domain of Bqt4 is a protein-interaction module that recognizes a consensus motif and plays essential roles in telomere-NE association and meiosis progression. Phosphorylation of Bqt4-interacting proteins may act as a switch to regulate these interactions during cell cycles. Our studies provide structural insights into the identification and regulation of Bqt4-mediated interactions.
收录类别	SCI ; SCIE
语种	英语
资助项目	International Cooperative Research Program of Institute for Protein Research, Osaka University[ICRa17-04]
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
WOS记录号	WOS:000467960500046
出版者	OXFORD UNIV PRESS
WOS关键词	ORGANIZATION ; GENOME ; RAP1 ; HETEROCHROMATIN ; ARCHITECTURE ; TELOMERES ; MODEL
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/40848
专题	生命科学与技术学院_硕士生生命科学与技术学院_特聘教授组_陈勇组
通讯作者	Kanoh, Junko; Chen, Yong
作者单位	1.Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, CAS Ctr Excellence Mol Cell Sci,Shanghai Sci Res, State Key Lab Mol Biol,Natl Ctr Prot Sci Shanghai, 333 Haike Rd, Shanghai 201210, Peoples R China 2.Univ Chinese Acad Sci, 333 Haike Rd, Shanghai 201210, Peoples R China 3.Osaka Univ, Inst Prot Res, 3-2 Yamadaoka, Suita, Osaka 5650871, Japan 4.Shanghai Tech Univ, Sch Life Sci & Technol, 100 Haike Rd, Shanghai 201210, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Hu, Chunyi,Inoue, Haruna,Sun, Wenqi,et al. Structural insights into chromosome attachment to the nuclear envelope by an inner nuclear membrane protein Bqt4 in fission yeast[J]. NUCLEIC ACIDS RESEARCH,2019,47(3):1573-1584.
APA	Hu, Chunyi.,Inoue, Haruna.,Sun, Wenqi.,Takeshita, Yumiko.,Huang, Yaoguang.,...&Chen, Yong.(2019).Structural insights into chromosome attachment to the nuclear envelope by an inner nuclear membrane protein Bqt4 in fission yeast.NUCLEIC ACIDS RESEARCH,47(3),1573-1584.
MLA	Hu, Chunyi,et al."Structural insights into chromosome attachment to the nuclear envelope by an inner nuclear membrane protein Bqt4 in fission yeast".NUCLEIC ACIDS RESEARCH 47.3(2019):1573-1584.