Unveiling tryptophan dynamics and functions across model organisms via quantitative imaging

doi:10.1186/s12915-024-02058-x

	Unveiling tryptophan dynamics and functions across model organisms via quantitative imaging
	Wang, Kui 1; Chen, Tian-lun 1; Zhang, Xin-xin1,3 ; Cao, Jian-bin 4; Wang, Pengcheng 1,5; Wang, Mingcang 4; Du, Jiu-lin1,2,3 ; Mu, Yu 1,2; Tao, Rongkun 1
	2024-11-14
发表期刊	BMC BIOLOGY (IF:4.4[JCR-2023],5.4[5-Year])
EISSN	1741-7007
卷号	22 期号:1
DOI	10.1186/s12915-024-02058-x
摘要	BackgroundTryptophan is an essential amino acid involved in critical cellular processes in vertebrates, serving as a precursor for serotonin and kynurenine, which are key neuromodulators to influence neural and immune functions. Systematic and quantitative measurement of tryptophan is vital to understanding these processes.ResultsHere, we utilized a robust and highly responsive green ratiometric indicator for tryptophan (GRIT) to quantitatively measure tryptophan dynamics in bacteria, mitochondria of mammalian cell cultures, human serum, and intact zebrafish. At the cellular scale, these quantitative analyses uncovered differences in tryptophan dynamics across cell types and organelles. At the whole-organism scale, we revealed that inflammation-induced tryptophan concentration increases in zebrafish brain led to elevated serotonin and kynurenine levels, prolonged sleep duration, suggesting a novel metabolic connection between immune response and behavior. Moreover, GRIT's application in detecting reduced serum tryptophan levels in patients with inflammation symptoms suggests its potential as a high-throughput diagnostic tool.ConclusionsIn summary, this study introduces GRIT as a powerful method for studying tryptophan metabolism and its broader physiological implications, paving the way for new insights into the metabolic regulation of health and disease across multiple biological scales.
关键词	Tryptophan quantitative imaging Bacteria Mitochondria Zebrafish Human serum
URL	查看原文
收录类别	PPRN.PPRN ; SCI
语种	英语
资助项目	National Science and Technology Innovation 2030 Major Program["2021ZD0202203","SQ2021AAA010882","2021ZD0204500","2021ZD0203704"] ; National Natural Science Foundation of China["32171090","32171026","32321003"] ; Shanghai Science and Technology Commission[21ZR1482600] ; Shanghai Municipal Science and Technology Major Project[2018SHZDZX05] ; The 2023 Youth Innovation Promotion Association CAS, Strategic Priority Research Program of the Chinese Academy of Sciences[XDA27010403] ; Scientific Instrument Developing Project of the Chinese Academy of Sciences[YJKYYQ20210029] ; Basic Public Welfare Research Project of Zhejiang Province[LGD20H090005]
WOS研究方向	Life Sciences & Biomedicine - Other Topics
WOS类目	Biology
WOS记录号	WOS:001353767800002
出版者	BMC
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/381289
专题	生命科学与技术学院生命科学与技术学院_特聘教授组_杜久林组生命科学与技术学院_博士生
通讯作者	Du, Jiu-lin; Mu, Yu; Tao, Rongkun
作者单位	1.Chinese Acad Sci, Inst Neurosci, Ctr Excellence Brain Sci & Intelligence Technol, State Key Lab Neurosci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China 2.Univ Chinese Acad Sci, 19A Yu Quan Rd, Beijing 100049, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, 319 Yue Yang Rd, Shanghai 200031, Peoples R China 4.Wenzhou Med Univ, Dept Anesthesiol, Taizhou Hosp Zhejiang Prov, 150 Xi Men Rd, Taizhou 317000, Zhejiang, Peoples R China 5.Shanghai Jiao Tong Univ, Sch Med, Dept Pediat Surg, Xinhua Hosp, 1665 Kong Jiang Rd, Shanghai 200092, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Wang, Kui,Chen, Tian-lun,Zhang, Xin-xin,et al. Unveiling tryptophan dynamics and functions across model organisms via quantitative imaging[J]. BMC BIOLOGY,2024,22(1).
APA	Wang, Kui.,Chen, Tian-lun.,Zhang, Xin-xin.,Cao, Jian-bin.,Wang, Pengcheng.,...&Tao, Rongkun.(2024).Unveiling tryptophan dynamics and functions across model organisms via quantitative imaging.BMC BIOLOGY,22(1).
MLA	Wang, Kui,et al."Unveiling tryptophan dynamics and functions across model organisms via quantitative imaging".BMC BIOLOGY 22.1(2024).