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Subtomogram averaging of helical filaments in cryo-electron tomography | |
2024-03-06 | |
状态 | 已发表 |
摘要 | Helical filaments are essential macromolecular elements in cellular organization and dynamics. Recent advances in cryo-electron tomography allow faithful imaging of isolated or in-cell helical filaments. Here, we present a protocol to generate density maps at sub-nanometer resolution of helical filaments by subtomogram averaging, exemplified with isolated mumps virus nucleocapsids and their in-cell form as an extension of the method. We detail procedures from pre-processing of tilt series frames to refinement of reconstructed averages for streamlined data processing of helical filaments. |
语种 | 英语 |
DOI | 10.1101/2024.03.04.583254 |
相关网址 | 查看原文 |
出处 | bioRxiv |
收录类别 | PPRN.PPRN |
WOS记录号 | PPRN:88026923 |
WOS类目 | Biophysics |
资助项目 | EMBL[3DCellPhase- 760067] |
文献类型 | 预印本 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/372986 |
专题 | iHuman研究所 iHuman研究所_PI研究组_张潇洁组 |
通讯作者 | Zhang, Xiaojie; Mahamid, Julia |
作者单位 | 1.Struct & Computat Biol Unit, European Mol Biol Lab, D-69117 Heidelberg, Baden Wurttemberg, Germany 2.ShanghaiTech Univ, iHuman Inst, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 3.ShanghaiTech Univ, Shanghai Key Lab High Resolut Electron Microscopy, Shanghai 201210, Peoples R China 4.Cell Biol & Biophys Unit, European Mol Biol Lab, D-69117 Heidelberg, Baden Wurttemberg, Germany |
推荐引用方式 GB/T 7714 | Zhang, Xiaojie,Mahamid, Julia. Subtomogram averaging of helical filaments in cryo-electron tomography. 2024. |
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