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| Subtomogram averaging of helical filaments in cryo-electron tomography | |
| 2024-03-06 | |
| 状态 | 已发表 |
| 摘要 | Helical filaments are essential macromolecular elements in cellular organization and dynamics. Recent advances in cryo-electron tomography allow faithful imaging of isolated or in-cell helical filaments. Here, we present a protocol to generate density maps at sub-nanometer resolution of helical filaments by subtomogram averaging, exemplified with isolated mumps virus nucleocapsids and their in-cell form as an extension of the method. We detail procedures from pre-processing of tilt series frames to refinement of reconstructed averages for streamlined data processing of helical filaments. |
| 语种 | 英语 |
| DOI | 10.1101/2024.03.04.583254 |
| 相关网址 | 查看原文 |
| 出处 | bioRxiv |
| 收录类别 | PPRN.PPRN |
| WOS记录号 | PPRN:88026923 |
| WOS类目 | Biophysics |
| 资助项目 | EMBL[3DCellPhase- 760067] |
| 文献类型 | 预印本 |
| 条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/372986 |
| 专题 | iHuman研究所 iHuman研究所_PI研究组_张潇洁组 |
| 通讯作者 | Zhang, Xiaojie; Mahamid, Julia |
| 作者单位 | 1.Struct & Computat Biol Unit, European Mol Biol Lab, D-69117 Heidelberg, Baden Wurttemberg, Germany 2.ShanghaiTech Univ, iHuman Inst, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 3.ShanghaiTech Univ, Shanghai Key Lab High Resolut Electron Microscopy, Shanghai 201210, Peoples R China 4.Cell Biol & Biophys Unit, European Mol Biol Lab, D-69117 Heidelberg, Baden Wurttemberg, Germany |
| 推荐引用方式 GB/T 7714 | Zhang, Xiaojie,Mahamid, Julia. Subtomogram averaging of helical filaments in cryo-electron tomography. 2024. |
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