Rapid Self-Assembly Mini-Livers Protect Mice Against Severe Hepatectomy-Induced Liver Failure

doi:10.1002/advs.202309166

	Rapid Self-Assembly Mini-Livers Protect Mice Against Severe Hepatectomy-Induced Liver Failure
	Miaomiao Luo, Jiahui Lai, Enhua Zhang, Yue Ma, Runbang He, Lina Mao, Bo Deng, Junjin Zhu, Yan Ding, Jialyu Huang, Bin Xue, Qiangsong Wang, Mingming Zhang, Pengyu Huang1,2
	2024-03
发表期刊	ADVANCED SCIENCE (IF:14.3[JCR-2023],16.3[5-Year])
ISSN	2198-3844
EISSN	2198-3844
卷号	11 期号:21
发表状态	已发表
DOI	10.1002/advs.202309166
摘要	The construction of bioartificial livers, such as liver organoids, offers significant promise for disease modeling, drug development, and regenerative medicine. However, existing methods for generating liver organoids have limitations, including lengthy and complex processes (taking 6–8 weeks or longer), safety concerns associated with pluripotency, limited functionality of pluripotent stem cell-derived hepatocytes, and small, highly variable sizes (typically ≈50–500 µm in diameter). Prolonged culture also leads to the formation of necrotic cores, further restricting size and function. In this study, a straightforward and time-efficient approach is developed for creating rapid self-assembly mini-livers (RSALs) within 12 h. Additionally, primary hepatocytes are significantly expanded in vitro for use as seeding cells. RSALs exhibit consistent larger sizes (5.5 mm in diameter), improved cell viability (99%), and enhanced liver functionality. Notably, RSALs are functionally vascularized within 2 weeks post-transplantation into the mesentery of mice. These authentic hepatocyte-based RSALs effectively protect mice from 90%-hepatectomy-induced liver failure, demonstrating the potential of bioartificial liver-based therapy. © 2024 The Authors. Advanced Science published by Wiley-VCH GmbH.
关键词	Artificial organs Self assembly Stem cells Acute liver injury Bioartificial liver Disease models Drug development Hepatocytes Liver failure Model drugs Organoids Rapid self assembly Regenerative medicine
收录类别	EI
语种	英语
出版者	John Wiley and Sons Inc
EI入藏号	20241215759905
EI主题词	Mammals
EI分类号	461.2 Biological Materials and Tissue Engineering ; 462.4 Prosthetics ; 951 Materials Science
原始文献类型	Article in Press
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/357316
专题	生命科学与技术学院_博士生
作者单位	1.State Key Laboratory of Advanced Medical Materials and Devices, Engineering Research Center of Pulmonary and Critical Care Medicine Technology and Device (Ministry of Education), Institute of Biomedical Engineering, Tianjin Institutes of Health Science, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin, 300192 China 2.School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210 China
第一作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Miaomiao Luo, Jiahui Lai, Enhua Zhang, Yue Ma, Runbang He, Lina Mao, Bo Deng, Junjin Zhu, Yan Ding, Jialyu Huang, Bin Xue, Qiangsong Wang, Mingming Zhang, Pengyu Huang. Rapid Self-Assembly Mini-Livers Protect Mice Against Severe Hepatectomy-Induced Liver Failure[J]. ADVANCED SCIENCE,2024,11(21).
APA	Miaomiao Luo, Jiahui Lai, Enhua Zhang, Yue Ma, Runbang He, Lina Mao, Bo Deng, Junjin Zhu, Yan Ding, Jialyu Huang, Bin Xue, Qiangsong Wang, Mingming Zhang, Pengyu Huang.(2024).Rapid Self-Assembly Mini-Livers Protect Mice Against Severe Hepatectomy-Induced Liver Failure.ADVANCED SCIENCE,11(21).
MLA	Miaomiao Luo, Jiahui Lai, Enhua Zhang, Yue Ma, Runbang He, Lina Mao, Bo Deng, Junjin Zhu, Yan Ding, Jialyu Huang, Bin Xue, Qiangsong Wang, Mingming Zhang, Pengyu Huang."Rapid Self-Assembly Mini-Livers Protect Mice Against Severe Hepatectomy-Induced Liver Failure".ADVANCED SCIENCE 11.21(2024).