Generation of functional posterior spinal motor neurons from hPSCs-derived human spinal cord neural progenitor cells

doi:10.1101/2022.06.26.495599

	Generation of functional posterior spinal motor neurons from hPSCs-derived human spinal cord neural progenitor cells
	Xu, J. H.1,7; Yao, Y.1,7; Yao, F.2; Chen, J.1,7; Li, M.3,7; Yang, X.1,4,7; Li, S.1,8; Lu, F.3; Hu, P.1,4,6,7,8; He, S.2 ; Peng, G.3,5,6,7; Jing, N.1,4,6,7
	2022-06-27
状态	已发表
摘要	Spinal motor neurons deficiency results in a series of devastating disorders such as amyotrophic lateral sclerosis (ALS), spinal muscular atrophy (SMA) and spinal cord injury (SCI). These disorders are currently incurable, while human pluripotent stem cells (hPSCs)-derived spinal motor neurons are promising but suffered from low-efficiency, functional immaturity and lacks of posterior cellular identity. In this study, we have established human spinal cord neural progenitor cells (hSCNPCs) via hPSCs differentiated neuromesodermal progenitors (NMPs) and demonstrated the hSCNPCs can be continuously expanded up to 40 passages. hSCNPCs can be rapidly differentiated into posterior spinal motor neurons with high efficiency. The functional maturity has been examined in detail. Moreover, a co-culture scheme which is compatible for both neural and muscular differentiation is developed to mimic the neuromuscular junction (NMJ) formation in vitro. Together, these studies highlight the potential avenues for generating clinically relevant spinal motor neurons and modeling neuromuscular diseases through our defined hSCNPCs.
语种	英语
DOI	10.1101/2022.06.26.495599
相关网址	查看原文
出处	bioRxiv
收录类别	PPRN.PPRN
WOS记录号	PPRN:9308801
WOS类目	Cell Biology
文献类型	预印本
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/348507
专题	生命科学与技术学院生命科学与技术学院_博士生
通讯作者	He, S.; Peng, G.; Jing, N.
作者单位	1.Shanghai Inst Biochem & Cell Biol, Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci, State Key Lab Cell Biol, Peoples R China 200031, Shanghai, China 2.ShanghaiTech Univ, Sch Life Sci & Technol, Peoples R China 201210, Shanghai, China 3.Guangzhou Inst Biomed & Hlth, Chinese Acad Sci, Ctr Cell Lineage & Dev, GIBH HKU Guangdong Hong Kong Stem Cell & Regenerat Med Res Ctr, Guangzhou 510530, Peoples R China 4.GuangzhouLaboratory, Guangzhou 510005, Peoples R China 5.Ctr Cell Lineage & Atlas, Bioland Lab, Guangzhou 510005, Peoples R China 6.Inst Stem Cell & Regenerat, Chinese Acad Sci, Peoples R China 100101, Beijing, China 7.Univ Chinese Acad Sci, Peoples R China, Beijing, China 8.Xinhua Hosp affiliated Shanghai Jiao Tong Univ Sch Med, 20023, Peoples R China, Shanghai, China
推荐引用方式 GB/T 7714	Xu, J. H.,Yao, Y.,Yao, F.,et al. Generation of functional posterior spinal motor neurons from hPSCs-derived human spinal cord neural progenitor cells. 2022.