WDR47 facilitates cell-specific ciliogenesis by modulating intraflagellar transport

doi:10.1101/2022.04.10.487758

	WDR47 facilitates cell-specific ciliogenesis by modulating intraflagellar transport
	Song, Chun-Xue 1,2; Zeng, Xian-Ting3 ; Zeng, Wan-Xin3 ; Tong, Xia-Jing3 ; Li, Q.1,2,4
	2022-04-10
状态	已发表
摘要	Cilia are conserved organelles found in many cell types in eukaryotes, and their dysfunction causes defects in environmental sensing and signaling transduction; such defects are termed ciliopathies. Distinct cilia have cell-specific morphologies and exert distinct functions. However, the underlying mechanisms of cell-specific ciliogenesis and regulation are unclear. Here we identified a WD40-repeat (WDR) protein, WDR47/NMTN-1, and show that it is specifically required for ciliogenesis of AWB chemosensory neurons in C. elegans. WDR47/NMTN-1 is expressed in the AWB chemosensory neuron pair, and is localized at the basal body (BB) of the AWB cilia. Knockout of wdr47/nmtn-1 causes abnormal AWB neuron cilia morphology, structural integrity, and induces aberrant AWB-mediated aversive behaviors. We further demonstrate that wdr47/nmtn-1 deletion affects movement of intraflagellar transport (IFT) particles and their cargo delivery in AWB neurons. Our results indicate that WDR47/NMTN-1 is essential for AWB neuron ciliary morphology and function, which reveal a novel mechanism for cell-specific ciliogenesis. Since WDR47/NMTN-1 is conserved in mammals, our findings may help understand the process of cell-specific ciliogenesis and provide insights for treating ciliopathies.
关键词	cilia chemosensory neuron WD40-repeat protein intraflagellar transport
语种	英语
DOI	10.1101/2022.04.10.487758
相关网址	查看原文
出处	bioRxiv
收录类别	PPRN.PPRN
WOS记录号	PPRN:8501695
WOS类目	Developmental Biology
资助项目	National Key Research and Development Program of China["32122038","21JC1404500","21SG16","QD2018017","ZDCX20211102","2021ZD0203100"] ; National Natural Science Foundation of China["31970933","19JC1414100"] ; Science and Technology Commission of Shanghai Municipality[32170963]
文献类型	预印本
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/348480
专题	生命科学与技术学院生命科学与技术学院_PI研究组_童夏静组生命科学与技术学院_博士生
通讯作者	Tong, Xia-Jing; Li, Q.
作者单位	1.Shanghai Jiao Tong Univ, Ctr Brain Sci, Shanghai Childrens Med Ctr, Sch Med, Shanghai 200127, Peoples R China 2.Shanghai Jiao Tong Univ, Shanghai Key Lab, Childrens Environm Hlth Xinhua Hosp, Dept Anat & Physiol, Minist Educ, Sch Med, Shanghai 200025, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 4.Shanghai Res Ctr Brain Sci & Brain Inspired Intelligence, Shanghai 201210, Peoples R China
推荐引用方式 GB/T 7714	Song, Chun-Xue,Zeng, Xian-Ting,Zeng, Wan-Xin,et al. WDR47 facilitates cell-specific ciliogenesis by modulating intraflagellar transport. 2022.