Dynamic elementomics of single-cell ICP-MS-derived signals in normal and calcium pump PMCA4-deficient mouse epididymal sperm during capacitation

doi:10.1093/mtomcs/mfad059

	Dynamic elementomics of single-cell ICP-MS-derived signals in normal and calcium pump PMCA4-deficient mouse epididymal sperm during capacitation
	Zhang, Bao Li1,2,3 ; Zhang, Ze Peng1 ; Shi, Su Meng1 ; Shi, Hui Juan 2; Deleon, Patricia A.4; Shum, Winnie1,2,3,5
	2023-10-04
发表期刊	METALLOMICS (IF:2.9[JCR-2023],3.7[5-Year])
ISSN	1756-5901
EISSN	1756-591X
卷号	15 期号:10
发表状态	已发表
DOI	10.1093/mtomcs/mfad059
摘要	["Currently, clinical analysis of male infertility mainly relies on parameters of semen and sperm cells. However, the high diagnostic failure rates indicate that the current assessment methods are not sufficient and a new approach to evaluating sperm function still needs to be developed. Here we explored the feasibility of single-cell inductively coupled plasma mass spectrometry (sc-ICP-MS)-derived profiles to determine the elemental characteristics in viable capacitated sperm under normal and deficient conditions. To validate the measurements, we used male sterile Pmca4-knockout (KO) mice with impaired calcium clearance, known to be dysregulated due to loss of calcium efflux capacity during sperm capacitation. Consistently, we observed significantly increased calcium intensities in Pmca4-KO sperm upon capacitation stimulation compared with control sperm from the caudaepididymides of wild-type control (WT) mice. More importantly, we explored that the characteristic signatures of calcium intensities in individual spikes derived from sc-ICP-MS was consistent with the dynamics of relative calcium levels in single sperm reported in the literature. Prominent alterations were also observed in the dynamic signatures of sc-ICP-MS-derived profiles of essential elements, particularly the redox-labile elements including copper, iron, manganese, selenium, and zinc in Pmca4-KO sperm compared to WT controls. Therefore, our study demonstrates that elementomics of sc-ICP-MS-derived signals can reveal ionic dysregulation in plasma membrane Ca2+-ATPase isoform 4 protein deficient sperm, and that sc-ICP-MS assay can be applied for functional analysis of viable sperm in functional activities, such as capacitation stimulation. We propose that cell elementomics can be used as an alternative approach to assessing sperm quality and male fertility at the single-cell level.","Graphical Abstract Dynamic sperm elementomics assessing male fertility."]
关键词	calcium pump PMCA4, cell elementomics elemental signal dynamics single-cell ICP-MS, sperm function assessment, sperm capacitation
URL	查看原文
收录类别	SCI ; EI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
WOS记录号	WOS:001082958100001
出版者	OXFORD UNIV PRESS
EI入藏号	20234414989699
EI主题词	Calcium
EI分类号	461.2 Biological Materials and Tissue Engineering ; 461.9 Biology ; 549.2 Alkaline Earth Metals ; 801 Chemistry
原始文献类型	Journal article (JA)
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/343587
专题	生命科学与技术学院生命科学与技术学院_PI研究组_岑慧枝组生命科学与技术学院_硕士生
通讯作者	Shum, Winnie
作者单位	1.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 2.Shanghai Inst Biomed & Pharmaceut Technol, Shanghai MOST Key Lab Hlth & Dis Genom, NHC Key Lab Reprod Regulat, Shanghai 200237, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Ctr Excellence Mol Cell Sci, Shanghai 200031, Peoples R China 4.Univ Delaware, Dept Biol Sci, Newark, DE 19716 USA 5.Shanghai Inst Biomed & Pharmaceut Technol, Shanghai 200032, Peoples R China
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
第一作者的第一单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Zhang, Bao Li,Zhang, Ze Peng,Shi, Su Meng,et al. Dynamic elementomics of single-cell ICP-MS-derived signals in normal and calcium pump PMCA4-deficient mouse epididymal sperm during capacitation[J]. METALLOMICS,2023,15(10).
APA	Zhang, Bao Li,Zhang, Ze Peng,Shi, Su Meng,Shi, Hui Juan,Deleon, Patricia A.,&Shum, Winnie.(2023).Dynamic elementomics of single-cell ICP-MS-derived signals in normal and calcium pump PMCA4-deficient mouse epididymal sperm during capacitation.METALLOMICS,15(10).
MLA	Zhang, Bao Li,et al."Dynamic elementomics of single-cell ICP-MS-derived signals in normal and calcium pump PMCA4-deficient mouse epididymal sperm during capacitation".METALLOMICS 15.10(2023).