Class I histone deacetylase complex: Structure and functional correlates

doi:10.1073/pnas.2307598120

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	Class I histone deacetylase complex: Structure and functional correlates
	Wang, Xiao1,2 ; Wang, Yannan1 ; Liu, Simiao 3; Zhang, Yi1 ; Xu, Ke1,2 ; Ji, Liting1,2 ; Kornberg, Roger D.1,4 ; Zhang, Heqiao1
	2023-07-17
发表期刊	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (IF:9.4[JCR-2023],10.8[5-Year])
ISSN	0027-8424
EISSN	1091-6490
卷号	120 期号:30
发表状态	已发表
DOI	10.1073/pnas.2307598120
摘要	["The Schizosaccharomyces pombe Clr6S complex, a class I histone deacetylase complex, functions as a zinc-dependent enzyme to remove acetyl groups from lysine residues in histone tails. We report here the cryo- EM structure of Clr6S alone and a cryo- EM map of Clr6S in complex with a nucleosome. The active center, revealed at near-atomic resolution, includes features important for catalysis-A water molecule coordinated by zinc, the likely nucleophile for attack on the acetyl-lysine bond, and a loop that may position the substrate for catalysis. The cryo- EM map in the presence of a nucleosome reveals multiple Clr6S-nucleosome contacts and a high degree of relative motion of Clr6S and the nucleosome. Such flexibility may be attributed to interaction at a site in the flexible histone tail and is likely important for the function of the deacetylase, which acts at multiple sites in other histone tails.","Significance","The fission yeast Clr6 HDAC complex is a large multisubunit histone deacetylase that removes acetyl groups from histone tails. Structures of catalytic subunits have been reported in the past. We report the cryo- EM structure of the entire Clr6S complex at 3.2 angstrom. The structure reveals two copies of a chromodomain-containing subunit and catalytic features, including a zinc ion and water molecule in the active center and a required protein loop nearby. We also report cryo- EM maps of a Clr6S- nucleosome complex and of the corresponding complex from Saccharomyces cerevisiae, which reveal mobility of the nucleosome important for catalytic activity."]
关键词	HDAC cryo-EM post translational modification deacetylase nucleosome
URL	查看原文
收录类别	SCI
语种	英语
资助项目	Shanxi Key Laboratory of Protein Structure Determination[202104010910006]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:001083463600004
出版者	NATL ACAD SCIENCES
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/331090
专题	免疫化学研究所生命科学与技术学院免疫化学研究所_特聘教授组_结构生物化学实验室生命科学与技术学院_博士生免疫化学研究所_公共科研平台_高通量筛选平台免疫化学研究所_PI研究组_Katsuhiko Mikoshiba组
通讯作者	Kornberg, Roger D.; Zhang, Heqiao
作者单位	1.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai 201210, Peoples R China 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 3.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China 4.Stanford Univ, Dept Biol Struct, Sch Med, Stanford, CA 94305 USA
第一作者单位	免疫化学研究所; 生命科学与技术学院
通讯作者单位	免疫化学研究所
第一作者的第一单位	免疫化学研究所
推荐引用方式 GB/T 7714	Wang, Xiao,Wang, Yannan,Liu, Simiao,et al. Class I histone deacetylase complex: Structure and functional correlates[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2023,120(30).
APA	Wang, Xiao.,Wang, Yannan.,Liu, Simiao.,Zhang, Yi.,Xu, Ke.,...&Zhang, Heqiao.(2023).Class I histone deacetylase complex: Structure and functional correlates.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,120(30).
MLA	Wang, Xiao,et al."Class I histone deacetylase complex: Structure and functional correlates".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 120.30(2023).