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ShanghaiTech University Knowledge Management System
Cellular population dynamics shape the route to human pluripotency | |
Panariello, Francesco1; Gagliano, Onelia2,3,4; Luni, Camilla5,6 ![]() ![]() ![]() ![]() ![]() ![]() | |
2023-05-17 | |
发表期刊 | NATURE COMMUNICATIONS (IF:14.7[JCR-2023],16.1[5-Year]) |
ISSN | 2041-1723 |
EISSN | 2041-1723 |
卷号 | 14期号:1 |
发表状态 | 已发表 |
DOI | 10.1038/s41467-023-37270-w |
摘要 | ["Human cellular reprogramming to induced pluripotency is still an inefficient process, which has hindered studying the role of critical intermediate stages. Here we take advantage of high efficiency reprogramming in microfluidics and temporal multi-omics to identify and resolve distinct sub-populations and their interactions. We perform secretome analysis and single-cell transcriptomics to show functional extrinsic pathways of protein communication between reprogramming sub-populations and the re-shaping of a permissive extracellular environment. We pinpoint the HGF/MET/STAT3 axis as a potent enhancer of reprogramming, which acts via HGF accumulation within the confined system of microfluidics, and in conventional dishes needs to be supplied exogenously to enhance efficiency. Our data suggest that human cellular reprogramming is a transcription factor-driven process that it is deeply dependent on extracellular context and cell population determinants.","The contribution of cell-extrinsic factors during cellular reprogramming to human induced pluripotent stem cells has long been overlooked. Here, the authors show functional protein communication between reprogramming intermediates and the re-shaping of a permissive extracellular environment."] |
URL | 查看原文 |
收录类别 | SCI |
语种 | 英语 |
资助项目 | Telethon Foundation Award[ |
WOS研究方向 | Science & Technology - Other Topics |
WOS类目 | Multidisciplinary Sciences |
WOS记录号 | WOS:001001374800004 |
出版者 | NATURE PORTFOLIO |
引用统计 | 正在获取...
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文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/321437 |
专题 | 免疫化学研究所 免疫化学研究所_特聘教授组_生物工程学实验室 免疫化学研究所_特聘教授组_干细胞生物学实验室 生命科学与技术学院_硕士生 免疫化学研究所_特聘教授组_张宏恺组 |
通讯作者 | Cacchiarelli, Davide; Elvassore, Nicola |
作者单位 | 1.Armenise Harvard Lab Integrat Genom, Telethon Inst Genet & Med TIGEM, Pozzuoli, Italy 2.Univ Padua, Dept Ind Engn, Padua, Italy 3.Veneto Inst Mol Med VIMM, Padua, Italy 4.UCL, GOS Inst Child Hlth, Stem Cell & Regenerat Med Sect, London, England 5.Shanghai Tech Univ, Shanghai Inst Adv Immunochem Studies, SIAIS, Shanghai, Peoples R China 6.Univ Bologna, Dept Civil Chem Environm & Mat Engn DICAM, Bologna, Italy 7.Univ Padua, Dept Informat Engn, Padua, Italy 8.Univ British Columbia, Dept Math, Vancouver, BC, Canada 9.Univ Padua, Dept Comparat Biomed & Food Sci, Padua, Italy 10.Univ Padua, CRIBI Biotechnol Ctr, Padua, Italy 11.Univ Naples Federico II, Dept Translat Med, Naples, Italy 12.Univ Naples Feder II, Sch Adv Studies, Genom & Expt Med Program, Naples, Italy 13.NEGEDIA Next Generat Diagnost srl, Pozzuoli, Italy |
通讯作者单位 | 免疫化学研究所 |
推荐引用方式 GB/T 7714 | Panariello, Francesco,Gagliano, Onelia,Luni, Camilla,et al. Cellular population dynamics shape the route to human pluripotency[J]. NATURE COMMUNICATIONS,2023,14(1). |
APA | Panariello, Francesco.,Gagliano, Onelia.,Luni, Camilla.,Grimaldi, Antonio.,Angiolillo, Silvia.,...&Elvassore, Nicola.(2023).Cellular population dynamics shape the route to human pluripotency.NATURE COMMUNICATIONS,14(1). |
MLA | Panariello, Francesco,et al."Cellular population dynamics shape the route to human pluripotency".NATURE COMMUNICATIONS 14.1(2023). |
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