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Cellular population dynamics shape the route to human pluripotency
2023-05-17
发表期刊NATURE COMMUNICATIONS (IF:14.7[JCR-2023],16.1[5-Year])
ISSN2041-1723
EISSN2041-1723
卷号14期号:1
发表状态已发表
DOI10.1038/s41467-023-37270-w
摘要

["Human cellular reprogramming to induced pluripotency is still an inefficient process, which has hindered studying the role of critical intermediate stages. Here we take advantage of high efficiency reprogramming in microfluidics and temporal multi-omics to identify and resolve distinct sub-populations and their interactions. We perform secretome analysis and single-cell transcriptomics to show functional extrinsic pathways of protein communication between reprogramming sub-populations and the re-shaping of a permissive extracellular environment. We pinpoint the HGF/MET/STAT3 axis as a potent enhancer of reprogramming, which acts via HGF accumulation within the confined system of microfluidics, and in conventional dishes needs to be supplied exogenously to enhance efficiency. Our data suggest that human cellular reprogramming is a transcription factor-driven process that it is deeply dependent on extracellular context and cell population determinants.","The contribution of cell-extrinsic factors during cellular reprogramming to human induced pluripotent stem cells has long been overlooked. Here, the authors show functional protein communication between reprogramming intermediates and the re-shaping of a permissive extracellular environment."]

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收录类别SCI
语种英语
资助项目Telethon Foundation Award[
WOS研究方向Science & Technology - Other Topics
WOS类目Multidisciplinary Sciences
WOS记录号WOS:001001374800004
出版者NATURE PORTFOLIO
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文献类型期刊论文
条目标识符https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/321437
专题免疫化学研究所
免疫化学研究所_特聘教授组_生物工程学实验室
免疫化学研究所_特聘教授组_干细胞生物学实验室
生命科学与技术学院_硕士生
免疫化学研究所_特聘教授组_张宏恺组
通讯作者Cacchiarelli, Davide; Elvassore, Nicola
作者单位
1.Armenise Harvard Lab Integrat Genom, Telethon Inst Genet & Med TIGEM, Pozzuoli, Italy
2.Univ Padua, Dept Ind Engn, Padua, Italy
3.Veneto Inst Mol Med VIMM, Padua, Italy
4.UCL, GOS Inst Child Hlth, Stem Cell & Regenerat Med Sect, London, England
5.Shanghai Tech Univ, Shanghai Inst Adv Immunochem Studies, SIAIS, Shanghai, Peoples R China
6.Univ Bologna, Dept Civil Chem Environm & Mat Engn DICAM, Bologna, Italy
7.Univ Padua, Dept Informat Engn, Padua, Italy
8.Univ British Columbia, Dept Math, Vancouver, BC, Canada
9.Univ Padua, Dept Comparat Biomed & Food Sci, Padua, Italy
10.Univ Padua, CRIBI Biotechnol Ctr, Padua, Italy
11.Univ Naples Federico II, Dept Translat Med, Naples, Italy
12.Univ Naples Feder II, Sch Adv Studies, Genom & Expt Med Program, Naples, Italy
13.NEGEDIA Next Generat Diagnost srl, Pozzuoli, Italy
通讯作者单位免疫化学研究所
推荐引用方式
GB/T 7714
Panariello, Francesco,Gagliano, Onelia,Luni, Camilla,et al. Cellular population dynamics shape the route to human pluripotency[J]. NATURE COMMUNICATIONS,2023,14(1).
APA Panariello, Francesco.,Gagliano, Onelia.,Luni, Camilla.,Grimaldi, Antonio.,Angiolillo, Silvia.,...&Elvassore, Nicola.(2023).Cellular population dynamics shape the route to human pluripotency.NATURE COMMUNICATIONS,14(1).
MLA Panariello, Francesco,et al."Cellular population dynamics shape the route to human pluripotency".NATURE COMMUNICATIONS 14.1(2023).
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