ShanghaiTech University Knowledge Management System
| Constructing a synthetic pathway for acetylcoenzyme A from one-carbon through enzyme design | |
Lu, Xiaoyun1; Liu, Yuwan1; Yang, Yiqun1,2; Wang, Shanshan3  ; Wang, Qian1,2; Wang, Xiya4; Yan, Zhihui1; Cheng, Jian1; Liu, Cui1; Yang, Xue1,2; Luo, Hao1,2; Yang, Sheng1; Gou, Junran1; Ye, Luzhen1; Lu, Lina1; Zhang, Zhidan1; Guo, Yu2,3,5; Nie, Yan3; Lin, Jianping1; Li, Sheng3; Tian, Chaoguang1; Cai, Tao1; Zhuo, Bingzhao6; Ma, Hongwu1; Wang, Wen6; Ma, Yanhe1; Liu, Yongjun4; Li, Yin7; Jiang, Huifeng1
| |
| 2019-03-26 | |
| Source Publication | NATURE COMMUNICATIONS
 |
| ISSN | 2041-1723 |
| Volume | 10 |
| Status | 已发表 |
| DOI | 10.1038/s41467-019-09095-z |
| Abstract | Acetyl-CoA is a fundamental metabolite for all life on Earth, and is also a key starting point for the biosynthesis of a variety of industrial chemicals and natural products. Here we design and construct a Synthetic Acetyl-CoA (SACA) pathway by repurposing glycolaldehyde synthase and acetyl-phosphate synthase. First, we design and engineer glycolaldehyde synthase to improve catalytic activity more than 70-fold, to condense two molecules of formaldehyde into one glycolaldehyde. Second, we repurpose a phosphoketolase to convert glycolaldehyde into acetyl-phosphate. We demonstrated the feasibility of the SACA pathway in vitro, achieving a carbon yield similar to 50%, and confirmed the SACA pathway by C-13-labeled metabolites. Finally, the SACA pathway was verified by cell growth using glycolaldehyde, formaldehyde and methanol as supplemental carbon source. The SACA pathway is proved to be the shortest, ATP-independent, carbon-conserving and oxygen-insensitive pathway for acetylCoA biosynthesis, opening possibilities for producing acetyl-CoA-derived chemicals from one-carbon resources in the future. |
| Indexed By | SCI ; SCIE |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[21773138] |
| WOS Research Area | Science & Technology - Other Topics |
| WOS Subject | Multidisciplinary Sciences |
| WOS ID | WOS:000462288800009 |
| Publisher | NATURE PUBLISHING GROUP |
| WOS Keyword | THIAMINE-DIPHOSPHATE ; MICROBIAL-PRODUCTION ; ESCHERICHIA-COLI ; MECHANISM ; PROTEIN ; ACTIVATION ; ENABLES ; ACID |
| Original Document Type | Article |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/30632 |
| Collection | 免疫化学研究所_特聘教授组_结构生物化学实验室 国际事务处 生命科学与技术学院_博士生 |
| Corresponding Author | Liu, Yongjun; Li, Yin; Jiang, Huifeng |
| Affiliation | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.ShanghaiTech Univ, Shanghai Inst Adv & Immunochem Studies, Shanghai 201210, Peoples R China 4.Shandong Univ, Sch Chem & Chem Engn, Jinan 250100, Shandong, Peoples R China 5.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 6.Northwestern Polytech Univ, Ctr Ecol & Environm Sci, Xian 710072, Shaanxi, Peoples R China 7.Chinese Acad Sci, Inst Microbiol, Key Lab Microbial Physiol & Metab Engn, State Key Lab Microbial Resources, Beijing 100101, Peoples R China |
| Recommended Citation GB/T 7714 | Lu, Xiaoyun,Liu, Yuwan,Yang, Yiqun,et al. Constructing a synthetic pathway for acetylcoenzyme A from one-carbon through enzyme design[J]. NATURE COMMUNICATIONS,2019,10. |
| APA | Lu, Xiaoyun.,Liu, Yuwan.,Yang, Yiqun.,Wang, Shanshan.,Wang, Qian.,...&Jiang, Huifeng.(2019).Constructing a synthetic pathway for acetylcoenzyme A from one-carbon through enzyme design.NATURE COMMUNICATIONS,10. |
| MLA | Lu, Xiaoyun,et al."Constructing a synthetic pathway for acetylcoenzyme A from one-carbon through enzyme design".NATURE COMMUNICATIONS 10(2019). |
| Files in This Item: | Download All | |||||
| File Name/Size | DocType | Version | Access | License | ||
Edit Comment
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.