| |||||||
ShanghaiTech University Knowledge Management System
Principles Governing Catalytic Activity of Self-Assembled Short Peptides | |
2019-01-09 | |
发表期刊 | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (IF:14.4[JCR-2023],14.8[5-Year]) |
ISSN | 0002-7863 |
卷号 | 141期号:1页码:223-231 |
发表状态 | 已发表 |
DOI | 10.1021/jacs.8b08893 |
摘要 | Molecular self-assembly provides a chemical strategy for the synthesis of nanostructures by using the principles of nature, and peptides serve as the promising building blocks to construct adaptable molecular architectures. Recently, a series of heptapeptides with alternative hydrophobic and hydrophilic residues were reported to form amyloid-like structures, which are capable of catalyzing acyl ester hydrolysis with remarkable efficiency. However, information remains elusive about the atomic structures of the fibrils. What is the origin of the sequence-dependent catalytic activity? How is the ester hydrolysis catalyzed by the fibrils? In this work, the atomic structures of the aggregates were determined by using molecular modeling and further validated by solid-state NMR experiments, where the fibril with high activity adopts twisted parallel configuration within each layer, and the one with low activity is in flat antiparallel configuration. The polymorphism originates from the interactions between different regions of the building block peptides, where the delicate balance between rigidity and flexibility plays an important role. We further show that the p-nitrophenylacetate (pNPA) hydrolysis reactions catalyzed by two different fibrils follow a similar mechanism, and the difference in microenvironment at the active site between the natural enzyme and the present self-assembled fibrils should account for the discrepancy in catalytic activities. The present work provides understanding of the structure and function of self-assembled fibrils formed with short peptides at an atomic level and thus sheds new insight on designing aggregates with better functions. |
收录类别 | SCI ; SCIE ; EI |
语种 | 英语 |
资助项目 | Fundamental Research Funds for the Central Universities[021514380012] ; Fundamental Research Funds for the Central Universities[021514380014] |
WOS研究方向 | Chemistry |
WOS类目 | Chemistry, Multidisciplinary |
WOS记录号 | WOS:000455561800039 |
出版者 | AMER CHEMICAL SOC |
WOS关键词 | CARBONIC-ANHYDRASE-II ; PROTON-TRANSFER ; SIDE-CHAINS ; BETA-SHEETS ; MECHANISM ; MODEL ; SIMULATIONS ; DYNAMICS ; COPPER ; PK(A) |
原始文献类型 | Article |
引用统计 | 正在获取...
|
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/30157 |
专题 | 生命科学与技术学院_博士生 生命科学与技术学院_PI研究组_陆珺霞组 生命科学与技术学院_公共科研平台_分子细胞学平台 |
通讯作者 | Wang, Wei; Lu, Junxia; Dong, Hao |
作者单位 | 1.Nanjing Univ, Kuang Yarning Honors Sch, Nanjing 210023, Jiangsu, Peoples R China 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 3.Nanjing Univ, Collaborat Innovat Ctr Adv Microstruct, Dept Phys, Natl Lab Solid State Microstruct, Nanjing 210093, Jiangsu, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Nanjing Univ, Inst Brain Sci, Nanjing 210023, Jiangsu, Peoples R China |
通讯作者单位 | 生命科学与技术学院 |
推荐引用方式 GB/T 7714 | Song, Ruiheng,Wu, Xialian,Xue, Bin,et al. Principles Governing Catalytic Activity of Self-Assembled Short Peptides[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2019,141(1):223-231. |
APA | Song, Ruiheng.,Wu, Xialian.,Xue, Bin.,Yang, Yuqin.,Huang, Wenmao.,...&Dong, Hao.(2019).Principles Governing Catalytic Activity of Self-Assembled Short Peptides.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,141(1),223-231. |
MLA | Song, Ruiheng,et al."Principles Governing Catalytic Activity of Self-Assembled Short Peptides".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 141.1(2019):223-231. |
条目包含的文件 | ||||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 |
修改评论
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。