Gene knockout of Zmym3 in mice arrests spermatogenesis at meiotic metaphase with defects in spindle assembly checkpoint

doi:10.1038/cddis.2017.228

	Gene knockout of Zmym3 in mice arrests spermatogenesis at meiotic metaphase with defects in spindle assembly checkpoint
	Hu, Xiangjing 1,2; Shen, Bin 3; Liao, Shangying 1; Ning, Yan 1,2; Ma, Longfei 1,2; Chen, Jian 1,3; Lin, Xiwen 1; Zhang, Daoqin 1,2; Li, Zhen 1,2; Zheng, Chunwei 1,2; Feng, Yanmin 1,3; Huang, Xingxu4 ; Han, Chunsheng 1
	2017-06
发表期刊	CELL DEATH & DISEASE
ISSN	2041-4889
卷号	8
发表状态	已发表
DOI	10.1038/cddis.2017.228
摘要	ZMYM3, a member of the MYM-type zinc finger protein family and a component of a LSD1-containing transcription repressor complex, is predominantly expressed in the mouse brain and testis. Here, we show that ZMYM3 in the mouse testis is expressed in somatic cells and germ cells until pachytene spermatocytes. Knockout (KO) of Zmym3 in mice using the CRISPR-Cas9 system resulted in adult male infertility. Spermatogenesis of the KO mice was arrested at the metaphase of the first meiotic division (MI). ZMYM3 co-immunoprecipitated with LSD1 in spermatogonial stem cells, but its KO did not change the levels of LSD1 or H3K4me1/2 or H3K9me2. However, Zmym3 KO resulted in elevated numbers of apoptotic germ cells and of MI spermatocytes that are positive for BUB3, which is a key player in spindle assembly checkpoint. Zmym3 KO also resulted in up-regulated expression of meiotic genes in spermatogonia. These results show that ZMYM3 has an essential role in metaphase to anaphase transition during mouse spermatogenesis by regulating the expression of diverse families of genes.
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31271379] ; National Natural Science Foundation of China[31471349]
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000405895200014
出版者	NATURE PUBLISHING GROUP
WOS关键词	LINKED MENTAL-RETARDATION ; SPERMATOGONIAL STEM-CELLS ; MALE GERM-CELLS ; RETINOIC ACID ; MEIOSIS ; PROGRESSION ; LSD1 ; EXPRESSION ; GENERATION ; CHROMATIN
原始文献类型	Article
通讯作者	Huang, Xingxu ; Han, Chunsheng
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2919
专题	生命科学与技术学院_PI研究组_黄行许组
通讯作者	Huang, Xingxu; Han, Chunsheng
作者单位	1.Chinese Acad Sci, Inst Zool, State Key Lab Stem Cell & Reprod Biol, 1-5 Bei Chen West Rd, Beijing 100101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Nanjing Med Univ, Dept Histol & Embryol, State Key Lab Reprod Med, Nanjing 210029, Jiangsu, Peoples R China 4.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Hu, Xiangjing,Shen, Bin,Liao, Shangying,et al. Gene knockout of Zmym3 in mice arrests spermatogenesis at meiotic metaphase with defects in spindle assembly checkpoint[J]. CELL DEATH & DISEASE,2017,8.
APA	Hu, Xiangjing.,Shen, Bin.,Liao, Shangying.,Ning, Yan.,Ma, Longfei.,...&Han, Chunsheng.(2017).Gene knockout of Zmym3 in mice arrests spermatogenesis at meiotic metaphase with defects in spindle assembly checkpoint.CELL DEATH & DISEASE,8.
MLA	Hu, Xiangjing,et al."Gene knockout of Zmym3 in mice arrests spermatogenesis at meiotic metaphase with defects in spindle assembly checkpoint".CELL DEATH & DISEASE 8(2017).