Norditerpenoids and Dinorditerpenoids from the Seeds of Podocarpus nagi as Cytotoxic Agents and Autophagy Inducers

doi:10.1021/acs.jnatprod.7b00347

	Norditerpenoids and Dinorditerpenoids from the Seeds of Podocarpus nagi as Cytotoxic Agents and Autophagy Inducers
	Feng, Zhe-Ling 1; Zhang, Le-Le 1; Zheng, Yuan-Dong2,3,4,5 ; Liu, Qian-Yu 1; Liu, Jing-Xin 1; Feng, Lu 2,3; Huang, Li 1; Zhang, Qing-Wen 1; Lu, Jin-Jian 1; Lin, Li-Gen 1
	2017-07
发表期刊	JOURNAL OF NATURAL PRODUCTS (IF:3.3[JCR-2023],3.9[5-Year])
ISSN	0163-3864
卷号	80 期号:7 页码:2110-2117
发表状态	已发表
DOI	10.1021/acs.jnatprod.7b00347
摘要	Nine new norditerpenoids and dinorditerpenoids, 2-oxonagilactone A (1), 7 beta-hydroxynagilactone D (2), nagilactones K and L (3 and 4), 3 beta-hydroxynagilactone L (5), 2 beta-hydroxynagilactone L (6), 3-epi-15-hydroxynagilactone D (7), l alpha-chloro-2 beta,3 beta,15-trihydroxynagilactone L (8), and 15-hydroxynagilactone L (9), were isolated from the seeds of Podocarpus nagi, along with eight known analogues. The structures of the new compounds were established based on detailed NMR and HRESIMS analysis, as well as from their ECD spectra. The absolute configuration of the known compound 1-deoxy-2 alpha-hydroxynagilactone A (16) was confirmed by single-crystal X-ray diffraction. All of the isolates were tested for their cytotoxic activities against cancer cells. The results indicated that compounds 4 and 6, as well as. several known compounds, displayed cytotoxicity against A2780 and HEY cancer cells. Among the new compounds, 2 beta-hydroxynagilactone L (6) showed IC50 values of less than 2.5 mu M against the two cell lines used. Furthermore, compound 6 induced autophagic flux in A2780 cells, as evidenced by an enhanced expression level of the autophagy marker phosphatidylethanolamine-i-nodified microtubule-associated protein light-chain 3 (LC3-II) and increased mRFP-GFP-LC3 puncta. Also, compound 6 activated the c-Jun N-terminal kinase (JNK) pathway, while pretreatment with the JNK inhibitor SP600125 decreased compound 6-induced autophagy.
收录类别	SCI ; IC
语种	英语
资助项目	Research Fund of University of Macau[MYRG2015-00153-ICMS-QRCM] ; Research Fund of University of Macau[MYRG2015-00091-ICMS-ARCM] ; Research Fund of University of Macau[MYRG2015-00101-ICMS-QRCM] ; Research Fund of University of Macau[MYRG2017-00109-ICMS]
WOS研究方向	Plant Sciences ; Pharmacology & Pharmacy
WOS类目	Plant Sciences ; Chemistry, Medicinal ; Pharmacology & Pharmacy
WOS记录号	WOS:000406818300019
出版者	AMER CHEMICAL SOC
WOS关键词	MACROPHYLLUS VAR.-MAKI ; CELL LUNG-CANCER ; C-JUN ; DILACTONES ; CHEMOTHERAPY ; NAGILACTONE ; ACTIVATION ; MATURATION ; PLANTS ; LC3
原始文献类型	Article
通讯作者	Lu, Jin-Jian ; Lin, Li-Gen
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2903
专题	生命科学与技术学院生命科学与技术学院_博士生
通讯作者	Lu, Jin-Jian; Lin, Li-Gen
作者单位	1.Univ Macau, Inst Chinese Med Sci, State Key Lab Qual Res Chinese Med, Ave Univ, Taipa 999078, Macao, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, 555 Zu Chong Zhi Rd,Zhangjiang Hi Tech Pk, Shanghai 201203, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Nat Prod Chem Dept, 555 Zu Chong Zhi Rd,Zhangjiang Hi Tech Pk, Shanghai 201203, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Feng, Zhe-Ling,Zhang, Le-Le,Zheng, Yuan-Dong,et al. Norditerpenoids and Dinorditerpenoids from the Seeds of Podocarpus nagi as Cytotoxic Agents and Autophagy Inducers[J]. JOURNAL OF NATURAL PRODUCTS,2017,80(7):2110-2117.
APA	Feng, Zhe-Ling.,Zhang, Le-Le.,Zheng, Yuan-Dong.,Liu, Qian-Yu.,Liu, Jing-Xin.,...&Lin, Li-Gen.(2017).Norditerpenoids and Dinorditerpenoids from the Seeds of Podocarpus nagi as Cytotoxic Agents and Autophagy Inducers.JOURNAL OF NATURAL PRODUCTS,80(7),2110-2117.
MLA	Feng, Zhe-Ling,et al."Norditerpenoids and Dinorditerpenoids from the Seeds of Podocarpus nagi as Cytotoxic Agents and Autophagy Inducers".JOURNAL OF NATURAL PRODUCTS 80.7(2017):2110-2117.