Dual genetic tracing reveals a unique fibroblast subpopulation modulating cardiac fibrosis

doi:10.1038/s41588-023-01337-7

	Dual genetic tracing reveals a unique fibroblast subpopulation modulating cardiac fibrosis
	Han, Maoying1,2 ; Liu, Zixin 1; Liu, Lei2 ; Huang, Xiuzhen 1; Wang, Haixiao 1; Pu, Wenjuan 1; Wang, Enci 3; Liu, Xiuxiu 1; Li, Yan 1; He, Lingjuan 4; Li, Xufeng 5; Wu, Jiayu 6; Qiu, Lin 6; Shen, Ruling 7; Wang, Qing-Dong 8; Ji, Yong 9; Ardehali, Reza 10; Shu, Qiang 11; Lui, Kathy O.12,13; Wang, Lixin 3,14; Zhou, Bin1,2,5,9,15
	2023-03-23
发表期刊	NAT GENETICS (IF:31.7[JCR-2023],36.6[5-Year])
ISSN	1061-4036
EISSN	1546-1718
发表状态	已发表
DOI	10.1038/s41588-023-01337-7
摘要	["After severe heart injury, fibroblasts are activated and proliferate excessively to form scarring, leading to decreased cardiac function and eventually heart failure. It is unknown, however, whether cardiac fibroblasts are heterogeneous with respect to their degree of activation, proliferation and function during cardiac fibrosis. Here, using dual recombinase-mediated genetic lineage tracing, we find that endocardium-derived fibroblasts preferentially proliferate and expand in response to pressure overload. Fibroblast-specific proliferation tracing revealed highly regional expansion of activated fibroblasts after injury, whose pattern mirrors that of endocardium-derived fibroblast distribution in the heart. Specific ablation of endocardium-derived fibroblasts alleviates cardiac fibrosis and reduces the decline of heart function after pressure overload injury. Mechanistically, Wnt signaling promotes activation and expansion of endocardium-derived fibroblasts during cardiac remodeling. Our study identifies endocardium-derived fibroblasts as a key fibroblast subpopulation accounting for severe cardiac fibrosis after pressure overload injury and as a potential therapeutic target against cardiac fibrosis.","Genetic lineage tracing in mice shows that endocardium-derived fibroblasts preferentially proliferate in response to pressure overload. Ablation of these cells alleviates cardiac fibrosis and reduces functional decline after pressure overload injury."]
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收录类别	SCI
语种	英语
资助项目	National Key Research & Development Program of China["2019YFA0110403","2019YFA0802000","2022YFA1104200","2021YFA0805100","2018YFA0108100"] ; National Science Foundation of China["82088101","32050087","82270415","81970412","32170848"] ; Shanghai Pilot Program for Basic Research-CAS, Shanghai Branch[JCYJ-SHFY-2021-0] ; CAS Project for Young Scientists in Basic Research[YSBR-012] ; Pearl River Talent Recruitment Program of Guangdong Province[2017ZT07S347] ; Research Grants Council of Hong Kong[RFS2223-4S04] ; Research Funds of Hangzhou Institute for Advanced Study[2022ZZ01015] ; Collaborative Innovation Program of Shanghai Municipal Health Commission[2020CXJQ01] ; Program of Shanghai Academic Research Leader[22XD1403700]
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
WOS记录号	WOS:000955758700002
出版者	NATURE PORTFOLIO
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/289984
专题	生命科学与技术学院生命科学与技术学院_特聘教授组_周斌组生命科学与技术学院_博士生
通讯作者	Zhou, Bin
作者单位	1.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Ctr Excellence Mol Cell Sci,State Key Lab Cell Bio, Shanghai, Peoples R China 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 3.Fudan Univ, Zhongshan Hosp, Dept Vasc Surg, Shanghai, Peoples R China 4.Westlake Univ, Sch Life Sci, Hangzhou, Peoples R China 5.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Life Sci, Key Lab Syst Hlth Sci Zhejiang Prov, Hangzhou, Peoples R China 6.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Nutr & Hlth, Key Lab Nutr Metab & Food Safety, Beijing, Peoples R China 7.Shanghai Lab Anim Res Ctr, Shanghai, Peoples R China 8.AstraZeneca, BioPharmaceut R&D, Biosci Cardiovasc Res & Early Dev Cardiovasc Renal, Gothenburg, Sweden 9.Nanjing Med Univ, Collaborat Innovat Ctr Cardiovasc Dis Translat Med, Nanjing, Peoples R China 10.Baylor Coll Med, Dept Med, Div Cardiol, Houston, TX USA 11.Zhejiang Univ, Childrens Hosp, Natl Clin Res Ctr Child Hlth, Sch Med,Dept Pediat Cardiaovasc Surg, Hangzhou, Peoples R China 12.Chinese Univ Hong Kong, Prince Wales Hosp, Dept Chem Pathol, Hong Kong, Peoples R China 13.Chinese Univ Hong Kong, Prince Wales Hosp, Li Ka Shing Inst Hlth Sci, Hong Kong, Peoples R China 14.Fudan Univ, Zhongshan Hosp Xiamen, Dept Vasc Surg, Xiamen, Peoples R China 15.New Cornerstone Sci Lab, Shenzhen, Peoples R China
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Han, Maoying,Liu, Zixin,Liu, Lei,et al. Dual genetic tracing reveals a unique fibroblast subpopulation modulating cardiac fibrosis[J]. NAT GENETICS,2023.
APA	Han, Maoying.,Liu, Zixin.,Liu, Lei.,Huang, Xiuzhen.,Wang, Haixiao.,...&Zhou, Bin.(2023).Dual genetic tracing reveals a unique fibroblast subpopulation modulating cardiac fibrosis.NAT GENETICS.
MLA	Han, Maoying,et al."Dual genetic tracing reveals a unique fibroblast subpopulation modulating cardiac fibrosis".NAT GENETICS (2023).