Cryo-EM Structure of the Human Ribonuclease P Holoenzyme

doi:10.1016/j.cell.2018.10.003

	Cryo-EM Structure of the Human Ribonuclease P Holoenzyme
	Wu, Jian 1; Niu, Shuangshuang2,3 ; Tan, Ming 2; Huang, Chenhui 1; Li, Mingyue2,3 ; Song, Yang 1; Wang, Qianmin 1; Chen, Juan 1; Shi, Shaohua 1; Lan, Pengfei 1; Lei, Ming 1,4,5,6
	2018-11-15
发表期刊	CELL (IF:45.5[JCR-2023],49.0[5-Year])
ISSN	0092-8674
卷号	175 期号:5 页码:1393-+
发表状态	已发表
DOI	10.1016/j.cell.2018.10.003
摘要	Ribonuclease (RNase) P is a ubiquitous ribozyme that cleaves the 5' leader from precursor tRNAs. Here, we report cryo-electron microscopy structures of the human nuclear RNase P alone and in complex with tRNA(val). Human RNase P is a large ribonucleoprotein complex that contains 10 protein components and one catalytic RNA. The protein components form an interlocked clamp that stabilizes the RNA in a conformation optimal for substrate binding. Human RNase P recognizes the tRNA using a double-anchor mechanism through both protein-RNA and RNA-RNA interactions. Structural comparison of the apo and tRNA-bound human RNase P reveals that binding of tRNA induces a local conformational change in the catalytic center, transforming the ribozyme into an active state. Our results also provide an evolutionary model depicting how auxiliary RNA elements in bacterial RNase P, essential for substrate binding, and catalysis, were replaced by the much more complex and multifunctional protein components in higher organisms.
收录类别	SCI ; SCIE
语种	英语
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDB08010201]
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Cell Biology
WOS记录号	WOS:000450328800021
出版者	CELL PRESS
WOS关键词	NUCLEAR RNASE P ; ESSENTIAL PROTEIN SUBUNIT ; PRECURSOR RIBOSOMAL-RNA ; DIVALENT METAL-ION ; CRYSTAL-STRUCTURE ; ACTIVE-SITE ; CONSERVED CORE ; BINDING-SITE ; C5 PROTEIN ; MRP
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/28700
专题	生命科学与技术学院_硕士生
通讯作者	Lan, Pengfei; Lei, Ming
作者单位	1.Shanghai Jiao Tong Univ, Shanghai Inst Precis Med, Peoples Hosp 9, Sch Med, Shanghai 200125, Peoples R China 2.Chinese Acad Sci, Univ Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, CAS Ctr Excellence Mol Cell Sci, Shanghai 200031, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 4.Shanghai Jiao Tong Univ, Key Lab Cell Differentiat & Apoptosis Chinese, Minist Educ, Sch Med, Shanghai 200025, Peoples R China 5.Zhangjiang Lab, Natl Facil Prot Sci, Shanghai 201210, Peoples R China 6.Chinese Acad Sci, Shanghai Sci Res Ctr, Shanghai 201204, Peoples R China
推荐引用方式 GB/T 7714	Wu, Jian,Niu, Shuangshuang,Tan, Ming,et al. Cryo-EM Structure of the Human Ribonuclease P Holoenzyme[J]. CELL,2018,175(5):1393-+.
APA	Wu, Jian.,Niu, Shuangshuang.,Tan, Ming.,Huang, Chenhui.,Li, Mingyue.,...&Lei, Ming.(2018).Cryo-EM Structure of the Human Ribonuclease P Holoenzyme.CELL,175(5),1393-+.
MLA	Wu, Jian,et al."Cryo-EM Structure of the Human Ribonuclease P Holoenzyme".CELL 175.5(2018):1393-+.