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| CRISPR/Cas9-based Genome Editing in Pseudomonas aeruginosa and Cytidine Deaminase-Mediated Base Editing in Pseudomonas Species | |
| 2018-08-31 | |
| Source Publication | ISCIENCE
 |
| ISSN | 2589-0042 |
| Volume | 6Pages:222-+ |
| Status | 已发表 |
| DOI | 10.1016/j.isci.2018.07.024 |
| Abstract | Pseudomonas species are a large class of gram-negative bacteria that exhibit significant biomedical, ecological, and industrial importance. Despite the extensive research and wide applications, genetic manipulation in Pseudomonas species, in particular in the major human pathogen Pseudomonas aeruginosa, remains a laborious endeavor. Here we report the development of a genome editing method pCasPA/pACRISPR by harnessing the CRISPR/Cas9 and the phage lambda-Red recombination systems. The method allows for efficient and scarless genetic manipulation in P. aeruginosa. By engineering the fusion of the cytidine deaminase APOBEC1 and the Cas9 nickase, we further develop a base editing system pnCasPA-BEC, which enables highly efficient gene inactivation and point mutations in a variety of Pseudomonas species, such as P. aeruginosa, Pseudomonas putida, Pseudomonas fluorescens, and Pseudomonas syringae. Application of the two genome editing methods will dramatically accelerate a wide variety of investigations, such as bacterial physiology study, drug target exploration, and metabolic engineering. |
| Indexed By | ESCI ; SCIE ; SCI |
| Language | 英语 |
| Funding Project | Shanghai Sailing Program[18YF1416500] |
| WOS Research Area | Science & Technology - Other Topics |
| WOS Subject | Multidisciplinary Sciences |
| WOS ID | WOS:000449733900016 |
| Publisher | CELL PRESS |
| WOS Keyword | STAPHYLOCOCCUS-AUREUS ; GUIDE RNA ; DNA ; PUTIDA ; SYSTEM ; EXPRESSION ; CAS9 ; RECOMBINATION ; ADAPTATION ; HOMOLOGY |
| Original Document Type | Article |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/28245 |
| Collection | 物质科学与技术学院_PI研究组_季泉江组 物质科学与技术学院_硕士生 物质科学与技术学院_博士生 |
| Corresponding Author | Ji, Quanjiang |
| Affiliation | Shanghai Tech Univ, Sch Phys Sci & Technol, Shanghai 201210, Peoples R China |
| First Author Affilication | School of Physical Science and Technology |
| Corresponding Author Affilication | School of Physical Science and Technology |
| First Signature Affilication | School of Physical Science and Technology |
| Recommended Citation GB/T 7714 | Chen, Weizhong,Zhang, Ya,Zhang, Yifei,et al. CRISPR/Cas9-based Genome Editing in Pseudomonas aeruginosa and Cytidine Deaminase-Mediated Base Editing in Pseudomonas Species[J]. ISCIENCE,2018,6:222-+. |
| APA | Chen, Weizhong.,Zhang, Ya.,Zhang, Yifei.,Pi, Yishuang.,Gu, Tongnian.,...&Ji, Quanjiang.(2018).CRISPR/Cas9-based Genome Editing in Pseudomonas aeruginosa and Cytidine Deaminase-Mediated Base Editing in Pseudomonas Species.ISCIENCE,6,222-+. |
| MLA | Chen, Weizhong,et al."CRISPR/Cas9-based Genome Editing in Pseudomonas aeruginosa and Cytidine Deaminase-Mediated Base Editing in Pseudomonas Species".ISCIENCE 6(2018):222-+. |
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