CRISPR/Cas9-based Genome Editing in Pseudomonas aeruginosa and Cytidine Deaminase-Mediated Base Editing in Pseudomonas Species
2018-08-31
Source PublicationISCIENCE
ISSN2589-0042
Volume6Pages:222-+
Status已发表
DOI10.1016/j.isci.2018.07.024
AbstractPseudomonas species are a large class of gram-negative bacteria that exhibit significant biomedical, ecological, and industrial importance. Despite the extensive research and wide applications, genetic manipulation in Pseudomonas species, in particular in the major human pathogen Pseudomonas aeruginosa, remains a laborious endeavor. Here we report the development of a genome editing method pCasPA/pACRISPR by harnessing the CRISPR/Cas9 and the phage lambda-Red recombination systems. The method allows for efficient and scarless genetic manipulation in P. aeruginosa. By engineering the fusion of the cytidine deaminase APOBEC1 and the Cas9 nickase, we further develop a base editing system pnCasPA-BEC, which enables highly efficient gene inactivation and point mutations in a variety of Pseudomonas species, such as P. aeruginosa, Pseudomonas putida, Pseudomonas fluorescens, and Pseudomonas syringae. Application of the two genome editing methods will dramatically accelerate a wide variety of investigations, such as bacterial physiology study, drug target exploration, and metabolic engineering.
Indexed ByESCI ; SCIE ; SCI
Language英语
Funding ProjectShanghai Sailing Program[18YF1416500]
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000449733900016
PublisherCELL PRESS
WOS KeywordSTAPHYLOCOCCUS-AUREUS ; GUIDE RNA ; DNA ; PUTIDA ; SYSTEM ; EXPRESSION ; CAS9 ; RECOMBINATION ; ADAPTATION ; HOMOLOGY
Original Document TypeArticle
Citation statistics
Document Type期刊论文
Identifierhttps://kms.shanghaitech.edu.cn/handle/2MSLDSTB/28245
Collection物质科学与技术学院_PI研究组_季泉江组
物质科学与技术学院_硕士生
物质科学与技术学院_博士生
Corresponding AuthorJi, Quanjiang
Affiliation
Shanghai Tech Univ, Sch Phys Sci & Technol, Shanghai 201210, Peoples R China
First Author AffilicationSchool of Physical Science and Technology
Corresponding Author AffilicationSchool of Physical Science and Technology
First Signature AffilicationSchool of Physical Science and Technology
Recommended Citation
GB/T 7714
Chen, Weizhong,Zhang, Ya,Zhang, Yifei,et al. CRISPR/Cas9-based Genome Editing in Pseudomonas aeruginosa and Cytidine Deaminase-Mediated Base Editing in Pseudomonas Species[J]. ISCIENCE,2018,6:222-+.
APA Chen, Weizhong.,Zhang, Ya.,Zhang, Yifei.,Pi, Yishuang.,Gu, Tongnian.,...&Ji, Quanjiang.(2018).CRISPR/Cas9-based Genome Editing in Pseudomonas aeruginosa and Cytidine Deaminase-Mediated Base Editing in Pseudomonas Species.ISCIENCE,6,222-+.
MLA Chen, Weizhong,et al."CRISPR/Cas9-based Genome Editing in Pseudomonas aeruginosa and Cytidine Deaminase-Mediated Base Editing in Pseudomonas Species".ISCIENCE 6(2018):222-+.
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