CRISPR/Cas9-based Genome Editing in Pseudomonas aeruginosa and Cytidine Deaminase-Mediated Base Editing in Pseudomonas Species

doi:10.1016/j.isci.2018.07.024

	CRISPR/Cas9-based Genome Editing in Pseudomonas aeruginosa and Cytidine Deaminase-Mediated Base Editing in Pseudomonas Species
	Chen, Weizhong; Zhang, Ya; Zhang, Yifei; Pi, Yishuang; Gu, Tongnian; Song, Liqiang; Wang, Yu; Ji, Quanjiang
	2018-08-31
发表期刊	ISCIENCE
ISSN	2589-0042
卷号	6 页码:222-+
发表状态	已发表
DOI	10.1016/j.isci.2018.07.024
摘要	Pseudomonas species are a large class of gram-negative bacteria that exhibit significant biomedical, ecological, and industrial importance. Despite the extensive research and wide applications, genetic manipulation in Pseudomonas species, in particular in the major human pathogen Pseudomonas aeruginosa, remains a laborious endeavor. Here we report the development of a genome editing method pCasPA/pACRISPR by harnessing the CRISPR/Cas9 and the phage lambda-Red recombination systems. The method allows for efficient and scarless genetic manipulation in P. aeruginosa. By engineering the fusion of the cytidine deaminase APOBEC1 and the Cas9 nickase, we further develop a base editing system pnCasPA-BEC, which enables highly efficient gene inactivation and point mutations in a variety of Pseudomonas species, such as P. aeruginosa, Pseudomonas putida, Pseudomonas fluorescens, and Pseudomonas syringae. Application of the two genome editing methods will dramatically accelerate a wide variety of investigations, such as bacterial physiology study, drug target exploration, and metabolic engineering.
收录类别	ESCI ; SCIE ; SCI
语种	英语
资助项目	Shanghai Sailing Program[18YF1416500]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000449733900016
出版者	CELL PRESS
WOS关键词	STAPHYLOCOCCUS-AUREUS ; GUIDE RNA ; DNA ; PUTIDA ; SYSTEM ; EXPRESSION ; CAS9 ; RECOMBINATION ; ADAPTATION ; HOMOLOGY
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/28245
专题	物质科学与技术学院_PI研究组_季泉江组物质科学与技术学院_硕士生物质科学与技术学院_博士生
通讯作者	Ji, Quanjiang
作者单位	Shanghai Tech Univ, Sch Phys Sci & Technol, Shanghai 201210, Peoples R China
第一作者单位	物质科学与技术学院
通讯作者单位	物质科学与技术学院
第一作者的第一单位	物质科学与技术学院
推荐引用方式 GB/T 7714	Chen, Weizhong,Zhang, Ya,Zhang, Yifei,et al. CRISPR/Cas9-based Genome Editing in Pseudomonas aeruginosa and Cytidine Deaminase-Mediated Base Editing in Pseudomonas Species[J]. ISCIENCE,2018,6:222-+.
APA	Chen, Weizhong.,Zhang, Ya.,Zhang, Yifei.,Pi, Yishuang.,Gu, Tongnian.,...&Ji, Quanjiang.(2018).CRISPR/Cas9-based Genome Editing in Pseudomonas aeruginosa and Cytidine Deaminase-Mediated Base Editing in Pseudomonas Species.ISCIENCE,6,222-+.
MLA	Chen, Weizhong,et al."CRISPR/Cas9-based Genome Editing in Pseudomonas aeruginosa and Cytidine Deaminase-Mediated Base Editing in Pseudomonas Species".ISCIENCE 6(2018):222-+.