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ShanghaiTech University Knowledge Management System
| Induction of human somatostatin and parvalbumin neurons by expressing a single transcription factor LIM homeobox 6 | |
| 2018-09-25 | |
| 发表期刊 | ELIFE (IF:6.4[JCR-2023],7.2[5-Year]) |
| ISSN | 2050-084X |
| 卷号 | 7 |
| 发表状态 | 已发表 |
| DOI | 10.7554/eLife.37382 |
| 摘要 | Human GABAergic interneurons (GIN) are implicated in normal brain function and in numerous mental disorders. However, the generation of functional human GIN subtypes from human pluripotent stem cells (hPSCs) has not been established. By expressing LHX6, a transcriptional factor that is critical for GIN development, we induced hPSCs to form GINs, including somatostatin (SST, 29%) and parvalbumin (PV, 21%) neurons. Our RNAseq results also confirmed the alteration of GIN identity with the overexpression of LHX6. Five months after transplantation into the mouse brain, the human GABA precursors generated increased population of SST and PV neurons by overexpressing LHX6. Importantly, the grafted human GINs exhibited functional electrophysiological properties and even fast-spiking-like action potentials. Thus, expression of the single transcription factor LHX6 under our GIN differentiation condition is sufficient to robustly induce human PV and SST subtypes. |
| 收录类别 | SCI ; SCIE |
| 语种 | 英语 |
| 资助项目 | Chinese Academy of Sciences[XDA16010306] |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics |
| WOS类目 | Biology |
| WOS记录号 | WOS:000446956400001 |
| 出版者 | ELIFE SCIENCES PUBLICATIONS LTD |
| WOS关键词 | PLURIPOTENT STEM-CELLS ; CORTICAL INTERNEURON FATE ; FUNCTIONAL MATURATION ; SONIC HEDGEHOG ; DIRECTED DIFFERENTIATION ; GABAERGIC NEURONS ; GENE-EXPRESSION ; SPECIFICATION ; LHX6 ; PROGENITORS |
| 原始文献类型 | Article |
| 引用统计 | 正在获取...
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| 文献类型 | 期刊论文 |
| 条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/28071 |
| 专题 | 生命科学与技术学院_博士生 生命科学与技术学院_PI研究组_何水金组 |
| 通讯作者 | He, Shuijin; Liu, Yan |
| 作者单位 | 1.Nanjing Med Univ, Sch Pharm, Inst Stem Cell & Neural Regenerat, Nanjing, Jiangsu, Peoples R China 2.Nanjing Med Unveristy, State Key Lab Reprod Med, Nanjing, Jiangsu, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 4.Nanjing Med Univ, Sch Basic Med Sci, Dept Neurosci, Nanjing, Jiangsu, Peoples R China 5.Fudan Univ, Inst Stem Cells & Regenerat Med, Dept Human Anat & Histol, Shanghai Med Sch, Shanghai, Peoples R China 6.Chinese Acad Sci, Inst Neurosci, Beijing, Peoples R China 7.Chinese Acad Sci, Inst Stem Cell & Regenerat, Beijing, Peoples R China |
| 通讯作者单位 | 生命科学与技术学院 |
| 推荐引用方式 GB/T 7714 | Yuan, Fang,Chen, Xin,Fang, Kai-Heng,et al. Induction of human somatostatin and parvalbumin neurons by expressing a single transcription factor LIM homeobox 6[J]. ELIFE,2018,7. |
| APA | Yuan, Fang.,Chen, Xin.,Fang, Kai-Heng.,Wang, Yuanyuan.,Lin, Mingyan.,...&Liu, Yan.(2018).Induction of human somatostatin and parvalbumin neurons by expressing a single transcription factor LIM homeobox 6.ELIFE,7. |
| MLA | Yuan, Fang,et al."Induction of human somatostatin and parvalbumin neurons by expressing a single transcription factor LIM homeobox 6".ELIFE 7(2018). |
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