A transgenic zebrafish model for in vivo long-term imaging of retinotectal synaptogenesis

doi:10.1038/s41598-018-32409-y

	A transgenic zebrafish model for in vivo long-term imaging of retinotectal synaptogenesis
	Du, Xu-fei 1; Xu, Bing 1; Zhang, Yu 1,2; Chen, Min-jia1,3 ; Du, Jiu-lin1,2,3
	2018-09-19
发表期刊	SCIENTIFIC REPORTS (IF:3.8[JCR-2023],4.3[5-Year])
ISSN	2045-2322
卷号	8
发表状态	已发表
DOI	10.1038/s41598-018-32409-y
摘要	The retinotectal synapse in larval zebrafish, combined with live time-lapse imaging, provides an advantageous model for study of the development and remodelling of central synapses in vivo. In previous studies, these synapses were labelled by transient expression of fluorescence-tagged synaptic proteins, which resulted in the dramatic variation of labelling patterns in each larva. Here, using GAL4-Upstream Activating Sequence (GAL4-UAS) methodology, we generated stable transgenic lines, which express EGFP-tagged synaptophysin (a presynaptic protein) in retinal ganglion cells (RGCs), to reliably label the pre-synaptic site of retinotectal synapses. This tool avoids the variable labelling of RGCs that occurs in transient transgenic larvae. We obtained several stable transgenic lines that differ consistently in the number of labelled RGCs. Using stable lines that consistently had a single labelled RGC, we could trace synaptogenic dynamics on an individual RGC axonal arbor across different developmental stages. In the stable lines that consistently had multiple labelled RGCs, we could simultaneously monitor both pre- and post-synaptic compartments by combining transient labelling of post-synaptic sites on individual tectal neurons. These tools allowed us to investigate molecular events underlying synaptogenesis and found that the microRNA-132 (miR-132) is required for developmental synaptogenesis. Thus, these transgenic zebrafish stable lines provide appropriate tools for studying central synaptogenesis and underlying molecular mechanisms in intact vertebrate brain.
收录类别	SCI ; SCIE
语种	英语
资助项目	Shanghai Science and Technology Committee[18JC1410100]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000445032500007
出版者	NATURE PUBLISHING GROUP
WOS关键词	NERVOUS-SYSTEM DEVELOPMENT ; SYNAPSE FORMATION ; NEURONAL-ACTIVITY ; NEURAL CIRCUITS ; VISUAL-CORTEX ; AXON GROWTH ; MECHANISMS ; MATURATION ; PLASTICITY ; DYNAMICS
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/27783
专题	生命科学与技术学院生命科学与技术学院_特聘教授组_杜久林组生命科学与技术学院_硕士生
通讯作者	Du, Xu-fei; Du, Jiu-lin
作者单位	1.Chinese Acad Sci, Inst Neurosci, State Key Lab Neurosci, Ctr Excellence Brain Sci & Intelligence Technol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China 2.Univ Chinese Acad Sci, Sch Future Technol, 19A Yu Quan Rd, Beijing 100049, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, 319 Yue Yang Rd, Shanghai 200031, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Du, Xu-fei,Xu, Bing,Zhang, Yu,et al. A transgenic zebrafish model for in vivo long-term imaging of retinotectal synaptogenesis[J]. SCIENTIFIC REPORTS,2018,8.
APA	Du, Xu-fei,Xu, Bing,Zhang, Yu,Chen, Min-jia,&Du, Jiu-lin.(2018).A transgenic zebrafish model for in vivo long-term imaging of retinotectal synaptogenesis.SCIENTIFIC REPORTS,8.
MLA	Du, Xu-fei,et al."A transgenic zebrafish model for in vivo long-term imaging of retinotectal synaptogenesis".SCIENTIFIC REPORTS 8(2018).