Enzymatic characterization and crystal structure of biosynthetic alanine racemase from Pseudomonas aeruginosa PAO1

doi:10.1016/j.bbrc.2018.06.155

	Enzymatic characterization and crystal structure of biosynthetic alanine racemase from Pseudomonas aeruginosa PAO1
	Dong, Hui 1,2; Han, Qingqing 3; Guo, Yu4,5,6 ; Ju, Jiansong 3; Wang, Shanshan4 ; Yuan, Chao4,5,6 ; Long, Wei 1,2; He, Xin 1,2; Xu, Shujing 3; Li, Sheng4
	2018-09-18
发表期刊	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (IF:2.5[JCR-2023],2.7[5-Year])
ISSN	0006-291X
卷号	503 期号:4 页码:2319-2325
发表状态	已发表
DOI	10.1016/j.bbrc.2018.06.155
摘要	Alanine racemase is a pyridoxal-5'-phosphate (PLP)-dependent enzyme that reversibly catalyzes the conversion of L-alanine to D-alanine. D-alanine is an essential constituent in many prokaryotic cell structures. Inhibition of alanine racemase is lethal to prokaryotes, creating an attractive target for designing antibacterial drugs. Here we report the crystal structure of biosynthetic alanine racemase (Alr) from a pathogenic bacteria Pseudomonas aeruginosa PAO1. Structural studies showed that P. aeruginosa Alr (PaAlr) adopts a conserved homodimer structure. A guest substrate D-lysine was observed in the active site and refined to dual-conformation. Two buffer ions, malonate and acetate, were bound in the proximity to D-lysine. Biochemical characterization revealed the optimal reaction conditions for PaAlr. (C) 2018 Elsevier Inc. All rights reserved.
关键词	Alanine racemase Pseudomonas aeruginosa PAO1 Biosynthetic Homodimer
收录类别	SCI ; SCIE
语种	英语
资助项目	Foundation of Hebei Educational Committee[ZD2017047]
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS类目	Biochemistry & Molecular Biology ; Biophysics
WOS记录号	WOS:000444791600019
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS关键词	D-AMINO ACIDS ; BACILLUS-STEAROTHERMOPHILUS ; EXTERNAL ALDIMINE ; ESCHERICHIA-COLI ; X-RAY ; GROWTH ; TUBERCULOSIS ; CYCLOSERINE ; RESOLUTION ; MECHANISM
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/27771
专题	生命科学与技术学院免疫化学研究所_特聘教授组_结构生物化学实验室生命科学与技术学院_博士生
通讯作者	Xu, Shujing; Li, Sheng
作者单位	1.Chinese Acad Med Sci, Inst Radiat Med, Key Lab Tianjin Radiat & Mol Nucl Med, Tianjin 300192, Peoples R China 2.Peking Union Med Coll, Tianjin 300192, Peoples R China 3.Hebei Normal Univ, Coll Life Sci, Shijiazhuang 050024, Hebei, Peoples R China 4.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai 201210, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
通讯作者单位	免疫化学研究所
推荐引用方式 GB/T 7714	Dong, Hui,Han, Qingqing,Guo, Yu,et al. Enzymatic characterization and crystal structure of biosynthetic alanine racemase from Pseudomonas aeruginosa PAO1[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2018,503(4):2319-2325.
APA	Dong, Hui.,Han, Qingqing.,Guo, Yu.,Ju, Jiansong.,Wang, Shanshan.,...&Li, Sheng.(2018).Enzymatic characterization and crystal structure of biosynthetic alanine racemase from Pseudomonas aeruginosa PAO1.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,503(4),2319-2325.
MLA	Dong, Hui,et al."Enzymatic characterization and crystal structure of biosynthetic alanine racemase from Pseudomonas aeruginosa PAO1".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 503.4(2018):2319-2325.