Apoptosis of Endothelial Cells Contributes to Brain Vessel Pruning of Zebrafish During Development

doi:10.3389/fnmol.2018.00222

	Apoptosis of Endothelial Cells Contributes to Brain Vessel Pruning of Zebrafish During Development
	Zhang, Yu 1,2; Xu, Bing 1,2; Chen, Qi 1; Yan, Yong1,3 ; Du, Jiulin1,2,3 ; Du, Xufei 1,2
	2018-06-28
发表期刊	FRONTIERS IN MOLECULAR NEUROSCIENCE
ISSN	1662-5099
卷号	11
发表状态	已发表
DOI	10.3389/fnmol.2018.00222
摘要	During development, immature blood vessel networks remodel to form a simplified and efficient vasculature to meet the demand for oxygen and nutrients, and this remodeling process is mainly achieved via the pruning of existing vessels. It has already known that the migration of vascular endothelial cells (ECs) is one of the mechanisms underlying vessel pruning. However, the role of EC apoptosis in vessel pruning remains under debate, especially in the brain. Here, we reported that EC apoptosis makes a significant contribution to vessel pruning in the brain of larval zebrafish. Using in vivo long-term time-lapse confocal imaging of the brain vasculature in zebrafish larvae, we found that EC apoptosis was always accompanied with brain vessel pruning and about 15% of vessel pruning events were resulted from EC apoptosis. In comparison with brain vessels undergoing EC migration-associated pruning, EC apoptosis-accompanied pruned vessels were longer and showed higher probability that the nuclei of neighboring vessels' ECs occupied their both ends. Furthermore, we found that microglia were responsible for the clearance of apoptotic ECs accompanying vessel pruning, though microglia themselves were dispensable for the occurrence of vessel pruning. Thus, our study demonstrates that EC apoptosis contributes to vessel pruning in the brain during development in a microglial cell-independent manner.
关键词	apoptosis endothelial cells vessel pruning microglia zebrafish
收录类别	SCI ; SCIE
语种	英语
资助项目	Yang-Fan Program from Science and Technology Commission of Shanghai Municipality[14YF1406600]
WOS研究方向	Neurosciences & Neurology
WOS类目	Neurosciences
WOS记录号	WOS:000436597600001
出版者	FRONTIERS MEDIA SA
WOS关键词	MICROGLIA ; ANGIOGENESIS ; DYNAMICS
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/27462
专题	生命科学与技术学院生命科学与技术学院_特聘教授组_杜久林组生命科学与技术学院_博士生
通讯作者	Du, Jiulin; Du, Xufei
作者单位	1.Chinese Acad Sci, Inst Neurosci, State Key Lab Neurosci, Ctr Excellence Brain Sci & Intelligence Technol, Shanghai, Peoples R China 2.Univ Chinese Acad Sci, Sch Future Technol, Beijing, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Zhang, Yu,Xu, Bing,Chen, Qi,et al. Apoptosis of Endothelial Cells Contributes to Brain Vessel Pruning of Zebrafish During Development[J]. FRONTIERS IN MOLECULAR NEUROSCIENCE,2018,11.
APA	Zhang, Yu,Xu, Bing,Chen, Qi,Yan, Yong,Du, Jiulin,&Du, Xufei.(2018).Apoptosis of Endothelial Cells Contributes to Brain Vessel Pruning of Zebrafish During Development.FRONTIERS IN MOLECULAR NEUROSCIENCE,11.
MLA	Zhang, Yu,et al."Apoptosis of Endothelial Cells Contributes to Brain Vessel Pruning of Zebrafish During Development".FRONTIERS IN MOLECULAR NEUROSCIENCE 11(2018).