CSAD Ameliorates Lipid Accumulation in High-Fat Diet-Fed Mice

doi:10.3390/ijms232415931

	CSAD Ameliorates Lipid Accumulation in High-Fat Diet-Fed Mice
	Tan, Rongrong 1,2; Li, Jiayang 1; Liu, Lu 2; Wu, Qian 2; Fan, Lei 2,3; Ma, Ningning2,4 ; Yu, Chuwei 2,3; Lu, Henglei 2; Zhang, Xuemei 1; Chen, Jing 2,3; Gong, Likun 2,3; Ren, Jin2,3,4
	2022-12-01
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
EISSN	1422-0067
卷号	23 期号:24
发表状态	已发表
DOI	10.3390/ijms232415931
摘要	Non-alcoholic fatty liver disease (NAFLD) is a chronic metabolic disease manifested in hepatic steatosis, inflammation, fibrosis, etc., which affects over one-quarter of the population around the world. Since no effective therapeutic drugs are available to cope with this widespread epidemic, the functional research of genes with altered expression during NAFLD helps understand the pathogenesis of this disease and the development of new potential therapeutic targets for drugs. In the current work, we discovered via the analysis of the Gene Expression Omnibus (GEO) dataset that cysteine sulfinic acid decarboxylase (CSAD) decreased significantly in NAFLD patients, which was also confirmed in multiple NAFLD mouse models (HFD-fed C57BL/6J, db/db and HFHFrHC-fed C57BL/6J mice). Next, CSAD's function in the progression of NAFLD was explored using AAV-mediated liver-directed gene overexpression in an HFD-fed mouse model, where the overexpression of CSAD in the liver could alleviate NAFLD-associated pathologies, including body weight, liver/body weight ratio, hepatic triglyceride and total cholesterol, and the degree of steatosis. Mechanically, we found that the overexpression of CSAD could increase the expression of some genes related to fatty acid beta-oxidation (Acad1, Ppara, and Acox1). Furthermore, we also detected that CSAD could improve mitochondrial injury in vitro and in vivo. Finally, we proposed that the effect of CSAD on lipid accumulation might be independent of the taurine pathway. In conclusion, we demonstrated that CSAD is involved in the development of NAFLD as a protective factor, which suggested that CSAD has the potential to become a new target for drug discovery in NAFLD.
关键词	NAFLD CSAD obesity liver damage fatty acid beta-oxidation mitochondrial damage
URL	查看原文
收录类别	SCI
语种	英语
资助项目	[ZYYCXTD-D-202210] ; [21DZ2291100]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:000901246100001
出版者	MDPI
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/268690
专题	生命科学与技术学院_博士生生命科学与技术学院_特聘教授组_任进组
通讯作者	Zhang, Xuemei; Ren, Jin
作者单位	1.Fudan Univ, Sch Pharm, Dept Pharmacol, 826 Zhangheng Rd, Shanghai 201203, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Safety Evaluat & Res, State Key Lab Drug Res, 501 Haike Rd, Shanghai 201203, Peoples R China 3.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China 4.Shanghai Tech Univ, Sch Life Sci & Technol, 100 Haike Rd, Shanghai 201210, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Tan, Rongrong,Li, Jiayang,Liu, Lu,et al. CSAD Ameliorates Lipid Accumulation in High-Fat Diet-Fed Mice[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2022,23(24).
APA	Tan, Rongrong.,Li, Jiayang.,Liu, Lu.,Wu, Qian.,Fan, Lei.,...&Ren, Jin.(2022).CSAD Ameliorates Lipid Accumulation in High-Fat Diet-Fed Mice.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,23(24).
MLA	Tan, Rongrong,et al."CSAD Ameliorates Lipid Accumulation in High-Fat Diet-Fed Mice".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 23.24(2022).