IL6 supports long-term expansion of hepatocytes in vitro

doi:10.1038/s41467-022-35167-8

	IL6 supports long-term expansion of hepatocytes in vitro
	Guo, Ren 1; Jiang, Mengmeng1,2,3 ; Wang, Gang 4; Li, Bing 1,3,5; Jia, Xiaohui 1,3; Ai, Yan 1,3,5; Chen, Shanshan 1,3; Tang, Peilan 1,6; Liu, Aijie 1; Yuan, Qianting 1; Xie, Xin1,2,3,4,5,7
	2022-11-29
发表期刊	NATURE COMMUNICATIONS (IF:14.7[JCR-2023],16.1[5-Year])
EISSN	2041-1723
卷号	13 期号:1
发表状态	已发表
DOI	10.1038/s41467-022-35167-8
摘要	Hepatocytes are very difficult to expand in vitro. A few studies have demonstrated that chemical cocktails with growth factors or Wnt ligands can support long-term expansion of hepatocytes via dedifferentiation. However, the culture conditions are complex, and clonal expansion of hepatic progenitors with full differentiation capacity are rarely reported. Here, we discover IL6, combined with EGF and HGF, promotes long-term expansion (>30 passages in similar to 150 days with theoretical expansion of similar to 10(35) times) of primary mouse hepatocytes in vitro in simple 2D culture, by converting hepatocytes into induced hepatic progenitor cells (iHPCs), which maintain the capacity of differentiation into hepatocytes. IL6 also supports the establishment of single hepatocyte-derived iHPC clones. The summation of the downstream STAT3, ERK and AKT pathways induces a number of transcription factors which support rapid growth. This physiological and simple way may provide ideas for culturing previously difficult-to-culture cell types and support their future applications.
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收录类别	SCI
语种	英语
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000890398000017
出版者	NATURE PORTFOLIO
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/268662
专题	生命科学与技术学院_博士生生命科学与技术学院_特聘教授组_谢欣组
通讯作者	Xie, Xin
作者单位	1.Chinese Acad Sci, Natl Ctr Drug Screening, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 3.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China 4.Fudan Univ, Sch Pharm, Dept Pharmaceut, Shanghai 201203, Peoples R China 5.Univ Chinese Acad Sci, Sch Pharmaceut Sci & Technol, Hangzhou Inst Adv Study, Hangzhou 310024, Peoples R China 6.Nanchang Univ, Sch Pharmaceut Sci, Nanchang 330006, Jiangxi, Peoples R China 7.Bohai Rim Adv Res Inst Drug Discovery, Shandong Lab Yantai Drug Discovery, Yantai 264117, Shandong, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Guo, Ren,Jiang, Mengmeng,Wang, Gang,et al. IL6 supports long-term expansion of hepatocytes in vitro[J]. NATURE COMMUNICATIONS,2022,13(1).
APA	Guo, Ren.,Jiang, Mengmeng.,Wang, Gang.,Li, Bing.,Jia, Xiaohui.,...&Xie, Xin.(2022).IL6 supports long-term expansion of hepatocytes in vitro.NATURE COMMUNICATIONS,13(1).
MLA	Guo, Ren,et al."IL6 supports long-term expansion of hepatocytes in vitro".NATURE COMMUNICATIONS 13.1(2022).