RNA granule-clustered mitochondrial aminoacyl-tRNA synthetases form multiple complexes with the potential to fine-tune tRNA aminoacylation

doi:10.1093/nar/gkac1141

	RNA granule-clustered mitochondrial aminoacyl-tRNA synthetases form multiple complexes with the potential to fine-tune tRNA aminoacylation
	Peng, Gui-Xin1,2 ; Mao, Xue-Ling 1; Cao, Yating 3; Yao, Shi-Ying 1; Li, Qing-Run 4; Chen, Xin 3; Wang, En-Duo1,2 ; Zhou, Xiao-Long 1
	2022-12-01
发表期刊	NUCLEIC ACIDS RESEARCH (IF:16.6[JCR-2023],16.1[5-Year])
ISSN	0305-1048
EISSN	1362-4962
发表状态	已发表
DOI	10.1093/nar/gkac1141
摘要	Mitochondrial RNA metabolism is suggested to occur in identified compartmentalized foci, i.e. mitochondrial RNA granules (MRGs). Mitochondrial aminoacyl-tRNA synthetases (mito aaRSs) catalyze tRNA charging and are key components in mitochondrial gene expression. Mutations of mito aaRSs are associated with various human disorders. However, the suborganelle distribution, interaction network and regulatory mechanism of mito aaRSs remain largely unknown. Here, we found that all mito aaRSs partly colocalize with MRG, and this colocalization is likely facilitated by tRNA-binding capacity. A fraction of human mitochondrial AlaRS (hmtAlaRS) and hmtSerRS formed a direct complex via interaction between catalytic domains in vivo. Aminoacylation activities of both hmtAlaRS and hmtSerRS were fine-tuned upon complex formation in vitro. We further established a full spectrum of interaction networks via immunoprecipitation and mass spectrometry for all mito aaRSs and discovered interactions between hmtSerRS and hmtAsnRS, between hmtSerRS and hmtTyrRS and between hmtThrRS and hmtArgRS. The activity of hmtTyrRS was also influenced by the presence of hmtSerRS. Notably, hmtSerRS utilized the same catalytic domain in mediating several interactions. Altogether, our results systematically analyzed the suborganelle localization and interaction network of mito aaRSs and discovered several mito aaRS-containing complexes, deepening our understanding of the functional and regulatory mechanisms of mito aaRSs.
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收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
WOS记录号	WOS:000896662700001
出版者	OXFORD UNIV PRESS
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/266589
专题	生命科学与技术学院_博士生生命科学与技术学院_特聘教授组_王恩多组
通讯作者	Wang, En-Duo; Zhou, Xiao-Long
作者单位	1.Univ Chinese Acad Sci, Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci, State Key Lab Mol Biol,Shanghai Inst Biochem & Cel, 320 Yue Yang Rd, Shanghai 200031, Peoples R China 2.Shanghai Tech Univ, Sch Life Sci & Technol, 393Middle Hua Xia Rd, Shanghai 201210, Peoples R China 3.Xiamen Univ, Fac Med & Life Sci, Sch Life Sci, State Key Lab Cellular Stress Biol, Xiamen 361102, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, CAS Ctr Excellence Mol Cell Sci, CAS Key Lab Syst Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Peng, Gui-Xin,Mao, Xue-Ling,Cao, Yating,et al. RNA granule-clustered mitochondrial aminoacyl-tRNA synthetases form multiple complexes with the potential to fine-tune tRNA aminoacylation[J]. NUCLEIC ACIDS RESEARCH,2022.
APA	Peng, Gui-Xin.,Mao, Xue-Ling.,Cao, Yating.,Yao, Shi-Ying.,Li, Qing-Run.,...&Zhou, Xiao-Long.(2022).RNA granule-clustered mitochondrial aminoacyl-tRNA synthetases form multiple complexes with the potential to fine-tune tRNA aminoacylation.NUCLEIC ACIDS RESEARCH.
MLA	Peng, Gui-Xin,et al."RNA granule-clustered mitochondrial aminoacyl-tRNA synthetases form multiple complexes with the potential to fine-tune tRNA aminoacylation".NUCLEIC ACIDS RESEARCH (2022).