上海科技大学知识管理系统

本发明涉及原代细胞体外培养领域，特别是涉及一种肝细胞培养基、培养方法及其用途。所述肝细胞培养基包括增殖培养基，所述增殖培养基包括以下成分：液体基础培养基、半乳糖、胰岛素‑转铁蛋白‑亚硒酸钠、脯氨酸、烟酰胺、鸟氨酸、转化生长因子α、表皮生长因子、地塞米松、Y‑27632、A‑83‑01、CHIR99021。本发明建立了原代肝细胞的长期体外扩增体系；肝细胞传代扩增培养时完全分化而不发生细胞退分化或转分化，降低了致癌风险，而且诱导成熟的肝细胞与原代肝细胞的肝功能差异小。

The invention relates to the field of culture of primary cells in vitro, in particular to a culture medium and culture method for hepatocytes and application of the hepatocytes. The culture medium forthe hepatocytes comprises a proliferation culture medium comprising the following components : a liquid basic culture medium, galactose, insulin-transferrin-sodium selenite, proline, niacinamide, ornithine, a transforming growth factor alpha, an epidermal growth factor, dexamethasone, Y-27632, A-83-01 and CHIR99021. A long-term in-vitro amplification system for primary hepatocytes is established; and the hepatocytes are completely differentiated without cell dedifferentiation or transdifferentiation during passage amplification culture, the carcinogenic risk is reduced, and the difference between hepatic functions of hepatocytes matured by induction and primary hepatocytes is small.