上海科技大学知识管理系统

Compositions and methods for detecting nucleic acid-protein interactions, or more generally interactions between a nucleic acid and another molecule. A Cas protein (e.g., a catalytically dead Cas13) is fused to a proximity tagging enzyme (e.g., a Pup ligase) and thus brings the proximity tagging enzyme to the proximity of a protein that binds to a nucleic acid, when the Cas protein recognizes the nucleic acid, e.g., through a guide RNA. The proximity tagging enzyme then tags the protein enabling it to be identified as a protein that interacts with the nucleic acid.

用于检测核酸-蛋白质相互作用，或更一般地检测核酸与另一分子之间的相互作用的组合物和方法。 Cas蛋白(例如，催化死亡的Cas13)与接近标签酶(例如，Pup连接酶)融合，并且因此当Cas蛋白例如通过指导RNA识别核酸时，使接近标签酶接近与核酸结合的蛋白质。 然后，接近标记酶标记蛋白质，使其能够被鉴定为与核酸相互作用的蛋白质。