Mechanistic Insights into Membrane Protein Clustering Revealed by Visualizing EGFR Secretion

doi:10.34133/2022/9835035

	Mechanistic Insights into Membrane Protein Clustering Revealed by Visualizing EGFR Secretion
	Xu, Haijiao 1; Zhang, Jinrui 1; Zhou, Yijia2,3 ; Zhao, Guanfang 1,4; Cai, Mingjun 1; Gao, Jing 1; Shao, Lina 1; Shi, Yan 1; Li, Hongru 1,4; Ji, Hongbin2,3,5,6 ; Zhao, Yikai 2; Wang, Hongda 1,4,7
	2022-10-19
发表期刊	RESEARCH (IF:8.5[JCR-2023],8.7[5-Year])
ISSN	2096-5168
EISSN	2639-5274
卷号	2022
发表状态	已发表
DOI	10.34133/2022/9835035
摘要	Most plasmalemmal proteins are organized into clusters to modulate various cellular functions. However, the machineries that regulate protein clustering remain largely unclear. Here, with EGFR as an example, we directly and in detail visualized the entire process of EGFR from synthesis to secretion onto the plasma membrane (PM) using a high-speed, high-resolution spinning-disk confocal microscope. First, colocalization imaging revealed that EGFR secretory vesicles underwent transport from the ER to the Golgi to the PM, eventually forming different distribution forms on the apical and basal membranes; that is, most EGFR formed larger clusters on the apical membrane than the basal membrane. A dynamic tracking image and further siRNA interference experiment confirmed that fusion of secretory vesicles with the plasma membrane led to EGFR clusters, and we showed that EGFR PM clustering may be intimately related to EGFR signaling and cell proliferation. Finally, we found that the size and origin of the secretory vesicles themselves may determine the difference in the distribution patterns of EGFR on the PM. More importantly, we showed that actin influenced the EGFR distribution by controlling the fusion of secretory vesicles with the PM. Collectively, a comprehensive understanding of the EGFR secretion process helps us to unravel the EGFR clustering process and elucidate the key factors determining the differences in the spatial distribution of EGFR PM, highlighting the correlation between EGFR secretion and its PM distribution pattern.
URL	查看原文
收录类别	SCI ; SCIE ; EI
语种	英语
资助项目	[22150003] ; [21727816] ; [21721003] ; [ZDKYYQ20220005] ; [YDZJ202201ZYTS530]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000889652900001
出版者	AMER ASSOC ADVANCEMENT SCIENCE
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/251698
专题	生命科学与技术学院_博士生生命科学与技术学院_特聘教授组_季红斌组
通讯作者	Zhao, Yikai; Wang, Hongda
作者单位	1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Ctr Excellence Mol Cell Sci, State Key Lab Cell Biol, Shanghai 200031, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 200031, Peoples R China 4.Univ Sci & Technol China, Hefei 230026, Anhui, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Life Sci, Hangzhou 130102, Peoples R China 7.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
推荐引用方式 GB/T 7714	Xu, Haijiao,Zhang, Jinrui,Zhou, Yijia,et al. Mechanistic Insights into Membrane Protein Clustering Revealed by Visualizing EGFR Secretion[J]. RESEARCH,2022,2022.
APA	Xu, Haijiao.,Zhang, Jinrui.,Zhou, Yijia.,Zhao, Guanfang.,Cai, Mingjun.,...&Wang, Hongda.(2022).Mechanistic Insights into Membrane Protein Clustering Revealed by Visualizing EGFR Secretion.RESEARCH,2022.
MLA	Xu, Haijiao,et al."Mechanistic Insights into Membrane Protein Clustering Revealed by Visualizing EGFR Secretion".RESEARCH 2022(2022).