Live-cell RNA imaging using the CRISPR-dCas13 system with modified sgRNAs appended with fluorescent RNA aptamers

doi:10.1039/d2sc04656c

	Live-cell RNA imaging using the CRISPR-dCas13 system with modified sgRNAs appended with fluorescent RNA aptamers
	Tang, Heng 1; Peng, Junran 1; Peng, Shuang 1; Wang, Qi 1; Jiang, Xin 1; Xue, Xiaocheng 1; Tao, Yanxin 2,3; Xiang, Limin 1; Ji, Quanjiang4 ; Liu, Song-Mei 2,3; Weng, Xiaocheng 1; Zhou, Xiang 1
	2022-11-08
发表期刊	CHEMICAL SCIENCE (IF:7.6[JCR-2023],8.0[5-Year])
ISSN	2041-6520
EISSN	2041-6539
发表状态	已发表
DOI	10.1039/d2sc04656c
摘要	The development of RNA imaging strategies in live cells is essential to improve our understanding of their role in various cellular functions. We report an efficient RNA imaging method based on the CRISPR-dPspCas13b system with fluorescent RNA aptamers in sgRNA (CasFAS) in live cells. Using modified sgRNA attached to fluorescent RNA aptamers that showed reduced background fluorescence, this approach provides a simple, sensitive way to image and track endogenous RNA with high accuracy and efficiency. In addition, color switching can be easily achieved by changing the fluorogenic dye analogues in living cells through user-friendly washing and restaining operations. CasFAS is compatible with orthogonal fluorescent aptamers, such as Broccoli and Pepper, enabling multiple colors RNA labeling or intracellular RNA-RNA interaction imaging. Finally, the visualization of severe fever with thrombocytopenia syndrome virus (SFTSV) was achieved by CasFAS, which may facilitate further studies on this virus.
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收录类别	SCI ; EI ; SCIE
语种	英语
资助项目	[21822704] ; [91940304] ; [21778040] ; [22177087] ; [91753201] ; [21721005] ; [91940000]
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
WOS记录号	WOS:000885106900001
出版者	ROYAL SOC CHEMISTRY
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/251692
专题	物质科学与技术学院_PI研究组_季泉江组
通讯作者	Weng, Xiaocheng; Zhou, Xiang
作者单位	1.Wuhan Univ, Inst Adv Studies, Coll Chem & Mol Sci, Key Lab Biomed Polymers,Minist Educ,Hubei Prov Key, Wuhan 430072, Hubei, Peoples R China 2.Wuhan Univ, Ctr Gene Diag, Dept Clin Lab, Zhongnan Hosp, Wuhan 430072, Hubei, Peoples R China 3.Wuhan Univ, Program Clin Lab Med, Zhongnan Hosp, Wuhan 430072, Hubei, Peoples R China 4.ShanghaiTech Univ, Sch Phys Sci & Technol, Shanghai 201210, Peoples R China
推荐引用方式 GB/T 7714	Tang, Heng,Peng, Junran,Peng, Shuang,et al. Live-cell RNA imaging using the CRISPR-dCas13 system with modified sgRNAs appended with fluorescent RNA aptamers[J]. CHEMICAL SCIENCE,2022.
APA	Tang, Heng.,Peng, Junran.,Peng, Shuang.,Wang, Qi.,Jiang, Xin.,...&Zhou, Xiang.(2022).Live-cell RNA imaging using the CRISPR-dCas13 system with modified sgRNAs appended with fluorescent RNA aptamers.CHEMICAL SCIENCE.
MLA	Tang, Heng,et al."Live-cell RNA imaging using the CRISPR-dCas13 system with modified sgRNAs appended with fluorescent RNA aptamers".CHEMICAL SCIENCE (2022).