Structural insights into Pot1-ssDNA, Pot1-Tpz1 and Tpz1-Ccq1 Interactions within fission yeast shelterin complex

doi:10.1371/journal.pgen.1010308

	Structural insights into Pot1-ssDNA, Pot1-Tpz1 and Tpz1-Ccq1 Interactions within fission yeast shelterin complex
	Sun, Hong1,2,3 ; Wu, Zhenfang 4,5; Zhou, Yuanze 6; Lu, Yanjia 4,5; Lu, Huaisheng 4,5; Chen, Hongwen 1,3; Shi, Shaohua 4,5; Zeng, Zhixiong 7; Wu, Jian 4,5; Lei, Ming 4,5,8,9
	2022-07
发表期刊	PLOS GENETICS (IF:4.0[JCR-2023],4.9[5-Year])
ISSN	1553-7404
卷号	18 期号:7
发表状态	已发表
DOI	10.1371/journal.pgen.1010308
摘要	The conserved shelterin complex caps chromosome ends to protect telomeres and regulate telomere replication. In fission yeast Schizosaccharomyces pombe, shelterin consists of telomeric single- and double-stranded DNA-binding modules Pot1-Tpz1 and Taz1-Rapt connected by Poz1, and a specific component Ccq1. While individual structures of the two DNA-binding OB folds of Pot1 (Pot1(O)(B1)-GGTTAC and Pot1(O)(B2)-GGITACGGT) are available, structural insight into recognition of telomeric repeats with spacers by the complete DNA-binding domain (Pot1(DBD)) remains an open question. Moreover, structural information about the Tpz1-Ccq1 interaction requires to be revealed for understanding how the specific component Ccq1 of S. pombe shelterin is recruited to telomeres to function as an interacting hub. Here, we report the crystal structures of Pot1(DBD)-single-stranded-DNA, Pot1(372-555)-TPZ1(185-212) and Tpz1(425-470)-Ccq1(123-439) complexes and propose an integrated model depicting the assembly mechanism of the shelterin complex at telomeres. The structure of Pot1(DBD)-DNA unveils how Pot1 recognizes S. pombe degenerate telomeric sequences. Our analyses of Tpz1-Ccq1 reveal structural basis for the essential role of the Tpz1-Ccq1 interaction in telomere recruitment of Ccq1 that is required for telomere maintenance and telomeric heterochromatin formation. Overall, our findings provide valuable structural information regarding interactions within fission yeast shelterin complex at 3' ss telomeric overhang.
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收录类别	SCI ; SCIE
语种	英语
资助项目	Ministry of Science and Technology of China[2018YFA0107004] ; National Natural Science Foundation of China["31930063","31270787"] ; Shanghai Municipal Education Commission-Gaofeng Clinical Medicine Grant Support[20181711]
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
WOS记录号	WOS:000886643100021
出版者	PUBLIC LIBRARY SCIENCE
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/251691
专题	生命科学与技术学院_博士生
通讯作者	Wu, Zhenfang; Zeng, Zhixiong; Wu, Jian; Lei, Ming
作者单位	1.Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, CAS Ctr Excellence Mol Cell Sci, State Key Lab Mol Biol, Shanghai, Peoples R China 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 3.Chinese Acad Sci, Univ Chinese Acad Sci, Beijing, Peoples R China 4.Shanghai Jiao Tong Univ, Ninth Peoples Hosp, Sch Med, Shanghai, Peoples R China 5.Shanghai Inst Precis Med, Shanghai, Peoples R China 6.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan, Peoples R China 7.Qilu Univ Technol, Shandong Prov Key Lab Microbial Engn, Coll Bioengn, Shandong, Peoples R China 8.Shanghai Jiao Tong Univ, State Key Lab Oncogenes & Related Genes, Sch Med, Shanghai, Peoples R China 9.Shanghai Jiao Tong Univ, Key Lab Cell Differentiat & Apoptosis Chinese Min, Sch Med, Shanghai, Peoples R China
第一作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Sun, Hong,Wu, Zhenfang,Zhou, Yuanze,et al. Structural insights into Pot1-ssDNA, Pot1-Tpz1 and Tpz1-Ccq1 Interactions within fission yeast shelterin complex[J]. PLOS GENETICS,2022,18(7).
APA	Sun, Hong.,Wu, Zhenfang.,Zhou, Yuanze.,Lu, Yanjia.,Lu, Huaisheng.,...&Lei, Ming.(2022).Structural insights into Pot1-ssDNA, Pot1-Tpz1 and Tpz1-Ccq1 Interactions within fission yeast shelterin complex.PLOS GENETICS,18(7).
MLA	Sun, Hong,et al."Structural insights into Pot1-ssDNA, Pot1-Tpz1 and Tpz1-Ccq1 Interactions within fission yeast shelterin complex".PLOS GENETICS 18.7(2022).