MARCKS regulates membrane targeting of Rab10 vesicles to promote axon development
Xu, Xiao-Hui1,2,3; Deng, Cai-Yun1,2; Liu, Yang1,2; He, Miao1,2; Peng, Jian1,2,4; Wang, Tong1,2; Yuan, Lei1,2; Zheng, Zhi-Sheng1,2; Blackshear, Perry J.5; Luo, Zhen-Ge1,2
2014-05
Source PublicationCELL RESEARCH
ISSN1001-0602
Volume24Issue:5Pages:576-594
Status已发表
DOI10.1038/cr.2014.33
Abstract

Axon development requires membrane addition from the intracellular supply, which has been shown to be mediated by Rab10-positive plasmalemmal precursor vesicles (PPVs). However, the molecular mechanisms underlying the membrane trafficking processes of PPVs remain unclear. Here, we show that myristoylated alanine-rich C-kinase substrate (MARCKS) mediates membrane targeting of Rab10-positive PPVs, and this regulation is critical for axon development. We found that the GTP-locked active form of Rab10 binds to membrane-associated MARCKS, whose affinity depends on the phosphorylation status of the MARCKS effector domain. Either genetic silencing of MARCKS or disruption of its interaction with Rab10 inhibited axon growth of cortical neurons, impaired docking and fusion of Rab10 vesicles with the plasma membrane, and consequently caused a loss of membrane insertion of axonal receptors responsive to extracellular axon growth factors. Thus, this study has identified a novel function of MARCKS in mediating membrane targeting of PPVs during axon development.

KeywordMARCKS Rab10 plasmalemmal precursor vesicles axon growth
Indexed BySCI
Funding ProjectNational Key Basic Research Program of China[2014CB910203]
WOS Research AreaCell Biology
WOS SubjectCell Biology
WOS IDWOS:000335914700006
PublisherINST BIOCHEMISTRY & CELL BIOLOGY
WOS KeywordPROTEIN-KINASE-C ; MAJOR SPECIFIC SUBSTRATE ; NEURONAL POLARIZATION ; PLASMA-MEMBRANE ; ANTEROGRADE TRANSPORT ; IN-VIVO ; PHOSPHORYLATION ; BRAIN ; GROWTH ; CELLS
Original Document TypeArticle
Citation statistics
Cited Times:35[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttps://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2404
Collection生命科学与技术学院_特聘教授组_罗振革组
生命科学与技术学院_硕士生
生命科学与技术学院_PI研究组_罗振革组
生命科学与技术学院_PI研究组_王彤组
Co-First AuthorDeng, Cai-Yun
Corresponding AuthorLuo, Zhen-Ge
Affiliation1.Chinese Acad Sci, Inst Neurosci, Shanghai 200031, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Neurosci, Shanghai 200031, Peoples R China
3.Shanghai Univ, Sch Life Sci, Shanghai 200444, Peoples R China
4.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 200031, Peoples R China
5.Natl Inst Environm Hlth Sci, Lab Signal Transduct, Res Triangle Pk, NC 27709 USA
Recommended Citation
GB/T 7714
Xu, Xiao-Hui,Deng, Cai-Yun,Liu, Yang,et al. MARCKS regulates membrane targeting of Rab10 vesicles to promote axon development[J]. CELL RESEARCH,2014,24(5):576-594.
APA Xu, Xiao-Hui.,Deng, Cai-Yun.,Liu, Yang.,He, Miao.,Peng, Jian.,...&Luo, Zhen-Ge.(2014).MARCKS regulates membrane targeting of Rab10 vesicles to promote axon development.CELL RESEARCH,24(5),576-594.
MLA Xu, Xiao-Hui,et al."MARCKS regulates membrane targeting of Rab10 vesicles to promote axon development".CELL RESEARCH 24.5(2014):576-594.
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