The Roles of Testicular C-kit Positive Cells in De novo Morphogenesis of Testis

doi:10.1038/srep05936

	The Roles of Testicular C-kit Positive Cells in De novo Morphogenesis of Testis
	Zhang, Man 1,2; Zhou, Hai 1,3; Zheng, Chunxing 4; Xiao, Jun 5; Zuo, Erwei 1,6; Liu, Wujuan 1; Xie, Da7 ; Shi, Yufang 4; Wu, Chunlian 3; Wang, Hongyan 5; Li, Dangsheng 8,9; Li, Jinsong1,7
	2014-08-04
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	4
发表状态	已发表
DOI	10.1038/srep05936
摘要	C-kit positive (c-kit(+)) cells are usual tissue-specific stem cells. However, in postnatal testis, undifferentiated spermatogonial stem cells (SSCs) are c-kit negative (c-kit(-)) and activation of c-kit represents the start of SSC differentiation, leaving an intriguing question whether other c-kit(+) cells exist and participate in the postnatal development of testis. To this end, a feasible system for testicular reconstitution, in which a specific type of cells can be manipulated, is needed. Here, we first establish de novo morphogenesis of testis by subcutaneous injection of testicular cells from neonatal testes into the backs of nude mice. We observe testicular tissue formation and spermatogenesis from all injected sites. Importantly, functional spermatids can be isolated from these testicular tissues. Using this system, we systemically analyze the roles of c-kit(+) cells in testicular reconstitution and identify a small population of cells (c-kit(+): CD140a(+): F4/80(+)), which express typical markers of macrophages, are critical for de novo morphogenesis of testis. Interestingly, we demonstrate that these cells are gradually replaced by peripheral blood cells of recipient mice during the morphogenesis of testis. Thus, we develop a system, which may mimic the complete developmental process of postnatal testis, for investigating the testicular development and spermatogenesis.
收录类别	SCI
语种	英语
资助项目	Shanghai Municipal Commission for Science and Technology[12JC1409600] ; Shanghai Municipal Commission for Science and Technology[13XD1404000]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000339941700002
出版者	NATURE PUBLISHING GROUP
WOS关键词	GERMLINE STEM-CELLS ; FACTOR RECEPTOR/C-KIT ; IN-VIVO ; MOUSE SPERMATOGENESIS ; SEMINIFEROUS TUBULES ; LEYDIG-CELLS ; W-LOCUS ; MACROPHAGES ; EXPRESSION ; SPERMATOGONIA
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2383
专题	生命科学与技术学院生命科学与技术学院_特聘教授组_李劲松组生命科学与技术学院_博士生
通讯作者	Li, Jinsong
作者单位	1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol,Grp Epigenet Reprogrammi, State Key Lab Cell Biol,Shanghai Key Lab Mol Andr, Shanghai 200031, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.China West Normal Univ, Coll Life Sci, Nanchong 637002, Sichuan, Peoples R China 4.Chinese Acad Sci, Shanghai Jiao Tong Univ, Sch Med,Shanghai Inst Biol Sci, Key Lab Stem Cell Biol,Inst Hlth Sci, Shanghai 200025, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Cell Biol, Shanghai 200031, Peoples R China 6.Guangxi Univ, Anim Reprod Inst, State Key Lab Conservat & Utilizat Subtrop Agrobi, Nanning 530004, Guangxi, Peoples R China 7.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai 200031, Peoples R China 8.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China 9.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Informat Ctr Life Sci, Shanghai 200031, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Zhang, Man,Zhou, Hai,Zheng, Chunxing,et al. The Roles of Testicular C-kit Positive Cells in De novo Morphogenesis of Testis[J]. SCIENTIFIC REPORTS,2014,4.
APA	Zhang, Man.,Zhou, Hai.,Zheng, Chunxing.,Xiao, Jun.,Zuo, Erwei.,...&Li, Jinsong.(2014).The Roles of Testicular C-kit Positive Cells in De novo Morphogenesis of Testis.SCIENTIFIC REPORTS,4.
MLA	Zhang, Man,et al."The Roles of Testicular C-kit Positive Cells in De novo Morphogenesis of Testis".SCIENTIFIC REPORTS 4(2014).