The genomic stability regulator PTIP is required for proper chromosome segregation in mitosis

doi:10.1186/s13008-022-00081-4

	The genomic stability regulator PTIP is required for proper chromosome segregation in mitosis
	Zhang, Fengxia 1; Wei, Mingxuan 1; Chen, Haoran 1; Ji, Liting2 ; Nie, Yan2 ; Kang, Jungseog 1,3
	2022-09-24
发表期刊	CELL DIVISION (IF:2.8[JCR-2023],3.1[5-Year])
EISSN	1747-1028
卷号	17 期号:1
发表状态	已发表
DOI	10.1186/s13008-022-00081-4
摘要	Background: The Pax transcription activation domain-interacting protein (PTIP) is a nuclear protein that is an essential component of H3K4 methylation for gene activation in vascular, kidney, B cell, and adipocyte development. Furthermore, it plays a key role in genomic stability in higher eukaryotic cells. It binds to 53BP1 and antagonizes inappropriate homologous recombination for a proper DNA damage response. Interestingly, an early study reported mitotic defects after PTIP inactivation, but it is not clear whether PTIP directly facilitates mitotic processes. Results: Here, we showed that PTIP is essential for the mitotic integrity of HeLa cells. PTIP inactivation increases cell death during mitotic exit, which appears to result from direct mitotic defects. PTIP inactivation did not affect the G2M DNA damage checkpoint during interphase upon etoposide treatment. However, in mitosis, PTIP inactivation results in prolonged mitotic time, inefficient chromosome alignment, and increased cell death. Furthermore, PTIP localizes to the mitotic centrosome via BRCT domains at the C-terminus. Conclusion: This study reveals a novel function of PTIP in maintaining the genomic stability of higher eukaryotes during mitosis. Therefore, its deregulation, which occurs in various tumors, may destabilize the genome by introducing an abnormal DNA damage response, as well as erroneous chromosome segregation.
关键词	Centrosome Chromosome segregation DNA damage Genetic instability Mitosis PTIP
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收录类别	SCIE
语种	英语
Scopus 记录号	2-s2.0-85138740752
来源库	Scopus
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/238183
专题	生命科学与技术学院_博士生免疫化学研究所国际事务处
通讯作者	Kang, Jungseog
作者单位	1.Arts and Science,New York University Shanghai,Shanghai,200122,China 2.Shanghai Institute for Advanced Immunochemical Studies,ShanghaiTech University,Shanghai,201210,China 3.NYU-ECNU Center for Computational Chemistry at NYU Shanghai,Shanghai,200062,China
推荐引用方式 GB/T 7714	Zhang, Fengxia,Wei, Mingxuan,Chen, Haoran,et al. The genomic stability regulator PTIP is required for proper chromosome segregation in mitosis[J]. CELL DIVISION,2022,17(1).
APA	Zhang, Fengxia,Wei, Mingxuan,Chen, Haoran,Ji, Liting,Nie, Yan,&Kang, Jungseog.(2022).The genomic stability regulator PTIP is required for proper chromosome segregation in mitosis.CELL DIVISION,17(1).
MLA	Zhang, Fengxia,et al."The genomic stability regulator PTIP is required for proper chromosome segregation in mitosis".CELL DIVISION 17.1(2022).