A minimalist mitochondrial threonyl-tRNA synthetase exhibits tRNA-isoacceptor specificity during proofreading

doi:10.1093/nar/gku1218

	A minimalist mitochondrial threonyl-tRNA synthetase exhibits tRNA-isoacceptor specificity during proofreading
	Zhou, Xiao-Long 1; Ruan, Zhi-Rong 1; Wang, Meng 1; Fang, Zhi-Peng 1; Wang, Yong2 ; Chen, Yun 1; Liu, Ru-Juan 1; Eriani, Gilbert 3; Wang, En-Duo1,2
	2014-12-16
发表期刊	NUCLEIC ACIDS RESEARCH
ISSN	0305-1048
卷号	42 期号:22 页码:13873-13886
发表状态	已发表
DOI	10.1093/nar/gku1218
摘要	Yeast mitochondria contain a minimalist threonyl-tRNA synthetase (ThrRS) composed only of the catalytic core and tRNA binding domain but lacking the entire editing domain. Besides the usual tRNA(Thr)2, some budding yeasts, such as Saccharomyces cerevisiae, also contain a non-canonical tRNA(Thr)1 with an enlarged 8-nucleotide anticodon loop, reprograming the usual leucine CUN codons to threonine. This raises interesting questions about the aminoacylation fidelity of such ThrRSs and the possible contribution of the two tRNA(Thr)s during editing. Here, we found that, despite the absence of the editing domain, S. cerevisiae mitochondrial ThrRS (ScmtThrRS) harbors a tRNA-dependent pre-transfer editing activity. Remarkably, only the usual tRNA(Thr)2 stimulated pre-transfer editing, thus, establishing the first example of a synthetase exhibiting tRNA-isoacceptor specificity during pre-transfer editing. We also showed that the failure of tRNAThr1 to stimulate tRNA-dependent pre-transfer editing was due to the lack of an editing domain. Using assays of the complementation of a ScmtThrRS gene knockout strain, we showed that the catalytic core and tRNA binding domain of ScmtThrRS co-evolved to recognize the unusual tRNA(Thr)1. In combination, the results provide insights into the tRNA-dependent editing process and suggest that tRNA-dependent pretransfer editing takes place in the aminoacylation catalytic core.
收录类别	SCI
语种	英语
资助项目	National Key Basic Research Foundation of China[2012CB911000]
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
WOS记录号	WOS:000347916900043
出版者	OXFORD UNIV PRESS
WOS关键词	PROTEIN-SYNTHESIS ; QUALITY-CONTROL ; SACCHAROMYCES-CEREVISIAE ; TRANSLATIONAL FIDELITY ; YEAST MITOCHONDRIA ; DOMAIN ; MECHANISM ; SEQUENCE ; ARCHAEA ; GENOME
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2349
专题	生命科学与技术学院_博士生生命科学与技术学院生命科学与技术学院_特聘教授组_王恩多组
通讯作者	Wang, En-Duo
作者单位	1.Chinese Acad Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai, Peoples R China 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 200031, Peoples R China 3.Univ Strasbourg, Architecture & Reactivite ARN, UPR9002, CNRS,Inst Biol Mol & Cellulaire, F-67084 Strasbourg, France
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Zhou, Xiao-Long,Ruan, Zhi-Rong,Wang, Meng,et al. A minimalist mitochondrial threonyl-tRNA synthetase exhibits tRNA-isoacceptor specificity during proofreading[J]. NUCLEIC ACIDS RESEARCH,2014,42(22):13873-13886.
APA	Zhou, Xiao-Long.,Ruan, Zhi-Rong.,Wang, Meng.,Fang, Zhi-Peng.,Wang, Yong.,...&Wang, En-Duo.(2014).A minimalist mitochondrial threonyl-tRNA synthetase exhibits tRNA-isoacceptor specificity during proofreading.NUCLEIC ACIDS RESEARCH,42(22),13873-13886.
MLA	Zhou, Xiao-Long,et al."A minimalist mitochondrial threonyl-tRNA synthetase exhibits tRNA-isoacceptor specificity during proofreading".NUCLEIC ACIDS RESEARCH 42.22(2014):13873-13886.