Helicase promotes replication re-initiation from an RNA transcript
2018-06-13
发表期刊NATURE COMMUNICATIONS
ISSN2041-1723
卷号9页码:2306
发表状态已发表
DOI10.1038/s41467-018-04702-x
摘要

To ensure accurate DNA replication, a replisome must effectively overcome numerous obstacles on its DNA substrate. After encountering an obstacle, a progressing replisome often aborts DNA synthesis but continues to unwind. However, little is known about how DNA synthesis is resumed downstream of an obstacle. Here, we examine the consequences of a non-replicating replisome collision with a co-directional RNA polymerase (RNAP). Using single-molecule and ensemble methods, we find that T7 helicase interacts strongly with a non-replicating T7 DNA polymerase (DNAP) at a replication fork. As the helicase advances, the associated DNAP also moves forward. The presence of the DNAP increases both helicase's processivity and unwinding rate. We show that such a DNAP, together with its helicase, is indeed able to actively disrupt a stalled transcription elongation complex, and then initiates replication using the RNA transcript as a primer. These observations exhibit T7 helicase's novel role in replication re-initiation.

收录类别SCI ; SCIE
资助项目National Science Foundation[MCB-1517764]
WOS研究方向Science & Technology - Other Topics
WOS类目Multidisciplinary Sciences
WOS记录号WOS:000435081300005
出版者NATURE PUBLISHING GROUP
WOS关键词BACTERIOPHAGE T7 REPLISOME ; LARGE-SCALE PURIFICATION ; LAGGING-STRAND SYNTHESIS ; DNA-REPLICATION ; SINGLE-MOLECULE ; FORK RESTART ; IN-VIVO ; POLYMERASE ; REVEALS ; MECHANISMS
原始文献类型Article
引用统计
文献类型期刊论文
条目标识符https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/23088
专题生命科学与技术学院_PI研究组_孙博组
通讯作者Sun, Bo; Wang, Michelle D.
作者单位
1.Cornell Univ, Howard Hughes Med Inst, Ithaca, NY 14853 USA
2.Cornell Univ, Phys Dept, Ithaca, NY 14853 USA
3.Cornell Univ, LASSP, Ithaca, NY 14853 USA
4.Rutgers State Univ, Robert Wood Johnson Med Sch, Dept Biochem Mol Biol, Piscataway, NJ 08854 USA
5.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
第一作者单位生命科学与技术学院
通讯作者单位生命科学与技术学院
推荐引用方式
GB/T 7714
Sun, Bo,Singh, Anupam,Sultana, Shemaila,et al. Helicase promotes replication re-initiation from an RNA transcript[J]. NATURE COMMUNICATIONS,2018,9:2306.
APA Sun, Bo,Singh, Anupam,Sultana, Shemaila,Inman, James T.,Patel, Smita S.,&Wang, Michelle D..(2018).Helicase promotes replication re-initiation from an RNA transcript.NATURE COMMUNICATIONS,9,2306.
MLA Sun, Bo,et al."Helicase promotes replication re-initiation from an RNA transcript".NATURE COMMUNICATIONS 9(2018):2306.
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