Protein arginine methyltransferase CARM1 attenuates the paraspeckle-mediated nuclear retention of mRNAs containing IRAlus

doi:10.1101/gad.257048.114

	Protein arginine methyltransferase CARM1 attenuates the paraspeckle-mediated nuclear retention of mRNAs containing IRAlus
	Hu, Shi-Bin 1; Xiang, Jian-Feng 1; Li, Xiang 1; Xu, Yefen 1; Xue, Wei 2; Huang, Min 1; Wong, Catharine C.1; Sagum, Cari A.3; Bedford, Mark T.3; Yang, Li2,4 ; Cheng, Donghang 3; Chen, Ling-Ling1,4
	2015-03-15
发表期刊	GENES & DEVELOPMENT
ISSN	0890-9369
卷号	29 期号:6 页码:630-645
发表状态	已发表
DOI	10.1101/gad.257048.114
摘要	In many cells, mRNAs containing inverted repeated Alu elements (IRAlus) in their 3' untranslated regions (UTRs) are inefficiently exported to the cytoplasm. Such nuclear retention correlates with paraspeckle-associated protein complexes containing p54(nrb). However, nuclear retention of mRNAs containing IRAlus is variable, and how regulation of retention and export is achieved is poorly understood. Here we show one mechanism of such regulation via the arginine methyltransferase CARM1 (coactivator-associated arginine methyltransferase 1). We demonstrate that disruption of CARM1 enhances the nuclear retention of mRNAs containing IRAlus. CARM1 regulates this nuclear retention pathway at two levels: CARM1 methylates the coiled-coil domain of p54nrb, resulting in reduced binding of p54nrb to mRNAs containing IRAlus, and also acts as a transcription regulator to suppress NEAT1 transcription, leading to reduced paraspeckle formation. These actions of CARM1 work together synergistically to regulate the export of transcripts containing IRAlus from paraspeckles under certain cellular stresses, such as poly(I:C) treatment. This work demonstrates how a post-translational modification of an RNA-binding protein affects protein-RNA interaction and also uncovers a mechanism of transcriptional regulation of the long noncoding RNA NEAT1.
关键词	paraspeckles p54(nrb) nuclear retention CARM1 NEAT1
收录类别	SCI
语种	英语
资助项目	National Institutes of Health[DK062248]
WOS研究方向	Cell Biology ; Developmental Biology ; Genetics & Heredity
WOS类目	Cell Biology ; Developmental Biology ; Genetics & Heredity
WOS记录号	WOS:000351556700006
出版者	COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
WOS关键词	LONG NONCODING RNA ; INVERTED REPEATS ; GENE-EXPRESSION ; BINDING PROTEIN ; METHYLATION ; TRANSCRIPTION ; BODIES ; DIFFERENTIATION ; ACTIVATION ; RELOCATION
原始文献类型	Article
引用统计	被引频次：75[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2243
专题	生命科学与技术学院_特聘教授组_杨力组生命科学与技术学院_特聘教授组_陈玲玲组
通讯作者	Yang, Li
作者单位	1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, German Max Planck Soc MPG Partner Inst Computat, Key Lab Computat Biol, Shanghai 200031, Peoples R China 3.Univ Texas MD Anderson Canc Ctr, Smithville, TX 78957 USA 4.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 200031, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Hu, Shi-Bin,Xiang, Jian-Feng,Li, Xiang,et al. Protein arginine methyltransferase CARM1 attenuates the paraspeckle-mediated nuclear retention of mRNAs containing IRAlus[J]. GENES & DEVELOPMENT,2015,29(6):630-645.
APA	Hu, Shi-Bin.,Xiang, Jian-Feng.,Li, Xiang.,Xu, Yefen.,Xue, Wei.,...&Chen, Ling-Ling.(2015).Protein arginine methyltransferase CARM1 attenuates the paraspeckle-mediated nuclear retention of mRNAs containing IRAlus.GENES & DEVELOPMENT,29(6),630-645.
MLA	Hu, Shi-Bin,et al."Protein arginine methyltransferase CARM1 attenuates the paraspeckle-mediated nuclear retention of mRNAs containing IRAlus".GENES & DEVELOPMENT 29.6(2015):630-645.