Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser

doi:10.1038/nature14656

KMS > iHuman研究所

	Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser
	Kang, Yanyong 1; Zhou, X. Edward 1; Gao, Xiang 1; He, Yuanzheng 1; Liu, Wei 2,3; Ishchenko, Andrii 4; Barty, Anton 5; Sathish, D.5; Yefanov, Oleksandr 5; Han, Gye Won 4; Xu, Qingping 6; de Waal, Parker W.1; Ke, Jiyuan 1; Tan, M. H. Eileen 1,7; Zhang, Chenghai 1; Moeller, Arne 8; West, Graham M.9; Pascal, Bruce D.9; Van Eps, Ned 10,11; Caro, Lydia N.12; Vishnivetskiy, Sergey A.13; Lee, Regina J.13; Suino-Powell, Kelly M.13; Gu, Xin 1; Pal, Kuntal 1; Ma, Jinming 1; Zhi, Xiaoyong 1; Boutet, Sebastien 14; Williams, Garth J.14; Messerschmidt, Marc 14,15; Gati, Cornelius 5; Zatsepin, Nadia A.2,3,16; Wang, Dingjie 2,3,16; James, Daniel 2,3,16; Basu, Shibom 2,3,16; Roy-Chowdhury, Shatabdi 2,3,16; Conrad, Chelsie E.2,3; Coe, Jesse 2,3; Liu, Haiguang 2,3,17; Lisova, Stella 2,3; Kupitz, Christopher 2,3,18; Grotjohann, Ingo 2,3; Fromme, Raimund 2,3; Jiang, Yi 19; Tan, Minjia 19; Yang, Huaiyu 19; Li, Jun 7; Wang, Meitian 20; Zheng, Zhong 21; Li, Dianfan 22; Howe, Nicole 22; Zhao, Yingming 15,23; Standfuss, Joerg 24; Diederichs, Kay 25; Dong, Yuhui 26; Potter, Clinton S.8; Carragher, Bridget 8; Caffrey, Martin 22; Jiang, Hualiang 19; Chapman, Henry N.5,27; Spence, John C. H.2,3,16; Fromme, Petra 2,3; Weierstall, Uwe 2,3,16; Ernst, Oliver P.12,28; Katritch, Vsevolod 21; Gurevich, Vsevolod V.13; Griffin, Patrick R.9; Hubbell, Wayne L.10,11; Stevens, Raymond C.4,21,29 ; Cherezov, Vadim 4; Melcher, Karsten 1; Xu, H. Eric 1,30
	2015-07-30
发表期刊	NATURE
ISSN	0028-0836
卷号	523 期号:7562 页码:561-+
发表状态	已发表
DOI	10.1038/nature14656
摘要	G-protein-coupled receptors (GPCRs) signal primarily through G proteins or arrestins. Arrestin binding to GPCRs blocks G protein interaction and redirects signalling to numerous G-protein-independent pathways. Here we report the crystal structure of a constitutively active form of human rhodopsin bound to a pre-activated form of the mouse visual arrestin, determined by serial femtosecond X-ray laser crystallography. Together with extensive biochemical and mutagenesis data, the structure reveals an overall architecture of the rhodopsin-arrestin assembly in which rhodopsin uses distinct structural elements, including transmembrane helix 7 and helix 8, to recruit arrestin. Correspondingly, arrestin adopts the pre-activated conformation, with a similar to 20 degrees rotation between the amino and carboxy domains, which opens up a cleft in arrestin to accommodate a short helix formed by the second intracellular loop of rhodopsin. This structure provides a basis for understanding GPCR-mediated arrestin-biased signalling and demonstrates the power of X-ray lasers for advancing the frontiers of structural biology.
收录类别	SCI
语种	英语
资助项目	National Institute of General Medical Sciences PSI: Biology grants[U54 GM094618] ; National Institute of General Medical Sciences PSI: Biology grants[GM108635] ; National Institute of General Medical Sciences PSI: Biology grants[U54 GM094599] ; National Institute of General Medical Sciences PSI: Biology grants[GM097463] ; National Institute of General Medical Sciences PSI: Biology grants[U54 GM094586]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000358655200038
出版者	NATURE PUBLISHING GROUP
WOS关键词	PROTEIN-COUPLED RECEPTOR ; LIPIDIC CUBIC PHASE ; ROD OUTER SEGMENTS ; VISUAL ARRESTIN ; SEROTONIN RECEPTORS ; THERMAL-STABILITY ; MEMBRANE-PROTEINS ; SPACE-GROUP ; ACTIVATION ; BINDING
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2171
专题	iHuman研究所 iHuman研究所_特聘教授组_Raymond Stevens组
通讯作者	Xu, H. Eric
作者单位	1.Van Andel Res Inst, Ctr Struct Biol & Drug Discovery, Lab Struct Sci, Grand Rapids, MI 49503 USA 2.Arizona State Univ, Dept Chem & Biochem, Biodesign Inst, Tempe, AZ 85287 USA 3.Arizona State Univ, Ctr Appl Struct Discovery, Biodesign Inst, Tempe, AZ 85287 USA 4.Univ So Calif, Bridge Inst, Dept Chem, Los Angeles, CA 90089 USA 5.Deutsch Elektronen Synchrotron DESY, Ctr Free Elect Laser Sci, D-22607 Hamburg, Germany 6.SLAC Natl Accelerator Lab, Stanford Synchrotron Radiat Lightsource, Joint Ctr Struct Genom, Menlo Pk, CA 94025 USA 7.Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Obstet & Gynecol, Singapore 117595, Singapore 8.New York Struct Biol Ctr, Natl Resource Automated Mol Microscopy, New York, NY 10027 USA 9.Scripps Florida, Scripps Res Inst, Dept Mol Therapeut, Jupiter, FL 33458 USA 10.Univ Calif Los Angeles, Jules Stein Eye Inst, Los Angeles, CA 90095 USA 11.Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USA 12.Univ Toronto, Dept Biochem, Toronto, ON M5S 1A8, Canada 13.Vanderbilt Univ, Dept Pharmacol, Nashville, TN 37232 USA 14.SLAC Natl Accelerator Lab, LCLS, Menlo Pk, CA 94025 USA 15.NSF Sci & Technol Ctr, BioXFEL, Buffalo, NY 14203 USA 16.Arizona State Univ, Dept Phys, Tempe, AZ 85287 USA 17.Beijing Computat Sci Res Ctr, Beijing 10084, Peoples R China 18.Univ Wisconsin, Dept Phys, Milwaukee, WI 53211 USA 19.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 20.Paul Scherrer Inst, Swiss Light Source, CH-5232 Villigen, Switzerland 21.Univ So Calif, Bridge Inst, Dept Biol Sci, Los Angeles, CA 90089 USA 22.Univ Dublin Trinity Coll, Sch Med & Sch Biochem & Immunol, Dublin 2, Ireland 23.Univ Chicago, Ben May Dept Canc Res, Chicago, IL 60637 USA 24.Paul Scherrer Inst, Lab Biomol Res, CH-5232 Villigen, Switzerland 25.Univ Konstanz, Dept Biol, D-78457 Constance, Germany 26.Chinese Acad Sci, Beijing Synchrotron Radiat Facil, Beijing 100049, Peoples R China 27.Ctr Ultrafast Imaging, D-22761 Hamburg, Germany 28.Univ Toronto, Dept Mol Genet, Toronto, ON M5S 1A8, Canada 29.ShanghaiTech Univ, iHuman Inst, Shanghai 201210, Peoples R China 30.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, VARI SIMM Ctr,Ctr Struct & Funct Drug Targets, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Kang, Yanyong,Zhou, X. Edward,Gao, Xiang,et al. Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser[J]. NATURE,2015,523(7562):561-+.
APA	Kang, Yanyong.,Zhou, X. Edward.,Gao, Xiang.,He, Yuanzheng.,Liu, Wei.,...&Xu, H. Eric.(2015).Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser.NATURE,523(7562),561-+.
MLA	Kang, Yanyong,et al."Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser".NATURE 523.7562(2015):561-+.