A threonyl-tRNA synthetase-like protein has tRNA aminoacylation and editing activities
2018-04-20
发表期刊NUCLEIC ACIDS RESEARCH
ISSN0305-1048
卷号46期号:7页码:3643-3656
发表状态已发表
DOI10.1093/nar/gky211
摘要TARS and TARS2 encode cytoplasmic and mitochondrial threonyl-tRNA synthetases (ThrRSs) in mammals, respectively. Interestingly, in higher eukaryotes, a third gene, TARSL2, encodes a ThrRS-like protein (ThrRS-L), which is highly homologous to cytoplasmic ThrRS but with a different N-terminal extension (N-extension). Whether ThrRS-L has canonical functions is unknown. In this work, we studied the organ expression pattern, cellular localization, canonical aminoacylation and editing activities of mouse ThrRS-L (mThrRS-L). Tarsl2 is ubiquitously but unevenly expressed in mouse tissues. Different from mouse cytoplasmic ThrRS (mThrRS), mThrRS-L is located in both the cytoplasm and nucleus; the nuclear distribution is mediated via a nuclear localization sequence at its C-terminus. Native mThrRS-L enriched from HEK293T cells was active in aminoacylation and editing. To investigate the in vitro catalytic properties of mThrRS-L accurately, we replaced the N-extension of mThrRS-L with that of mThrRS. The chimeric protein (mThrRS-L-NT) has amino acid activation, aminoacylation and editing activities. We compared the activities and cross-species tRNA recognition between mThrRS-L-NT and mThrRS. Despite having a similar aminoacylation activity, mThrRS-L-NT and mThrRS exhibit differences in tRNA recognition and editing capacity. Our results provided the first analysis of the aminoacylation and editing activities of ThrRS-L, and improved our understanding of Tarsl2.
收录类别SCI ; SCIE
语种英语
资助项目China Postdoctoral Science Foundation[2014T70438]
WOS研究方向Biochemistry & Molecular Biology
WOS类目Biochemistry & Molecular Biology
WOS记录号WOS:000431137900034
出版者OXFORD UNIV PRESS
WOS关键词TRANSLATIONAL QUALITY-CONTROL ; ACTIVE-SITE ; AMINO-ACIDS ; DOMAIN ; TRNA(LEU) ; NUCLEAR ; DISCRIMINATION ; BIOSYNTHESIS ; MECHANISM ; FIDELITY
原始文献类型Article
引用统计
文献类型期刊论文
条目标识符https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/20855
专题生命科学与技术学院
生命科学与技术学院_特聘教授组_王恩多组
生命科学与技术学院_博士生
通讯作者Zhou, Xiao-Long; Wang, En-Duo
作者单位
1.Univ Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem & Cell Biol, State Key Lab Mol Biol,Chinese Acad Sci, 320 Yue Yang Rd, Shanghai, Peoples R China
2.ShanghaiTech Univ, Sch Life Sci & Technol, 100 Haike Rd, Shanghai, Peoples R China
通讯作者单位生命科学与技术学院
推荐引用方式
GB/T 7714
Chen, Yun,Ruan, Zhi-Rong,Wang, Yong,et al. A threonyl-tRNA synthetase-like protein has tRNA aminoacylation and editing activities[J]. NUCLEIC ACIDS RESEARCH,2018,46(7):3643-3656.
APA Chen, Yun.,Ruan, Zhi-Rong.,Wang, Yong.,Huang, Qian.,Xue, Mei-Qin.,...&Wang, En-Duo.(2018).A threonyl-tRNA synthetase-like protein has tRNA aminoacylation and editing activities.NUCLEIC ACIDS RESEARCH,46(7),3643-3656.
MLA Chen, Yun,et al."A threonyl-tRNA synthetase-like protein has tRNA aminoacylation and editing activities".NUCLEIC ACIDS RESEARCH 46.7(2018):3643-3656.
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