Somatosensory neuron types identified by high-coverage single-cell RNA-sequencing and functional heterogeneity

doi:10.1038/cr.2015.149

	Somatosensory neuron types identified by high-coverage single-cell RNA-sequencing and functional heterogeneity
	Li, Chang-Lin 1,2; Li, Kai-Cheng 1,2; Wu, Dan 1,2; Chen, Yan 1,2; Luo, Hao5 ; Zhao, Jing-Rong 1,2; Wang, Sa-Shuang5 ; Sun, Ming-Ming 3; Lu, Ying-Jin 1,2; Zhong, Yan-Qing 1,2; Hu, Xu-Ye 6; Hou, Rui 3; Zhou, Bei-Bei 3; Bao, Lan4,5 ; Xiao, Hua-Sheng 3; Zhang, Xu1,2,5
	2016-01
发表期刊	CELL RESEARCH (IF:28.1[JCR-2023],36.4[5-Year])
ISSN	1001-0602
卷号	26 期号:1 页码:83-102
发表状态	已发表
DOI	10.1038/cr.2015.149
摘要	Sensory neurons are distinguished by distinct signaling networks and receptive characteristics. Thus, sensory neuron types can be defined by linking transcriptome-based neuron typing with the sensory phenotypes. Here we classify somatosensory neurons of the mouse dorsal root ganglion (DRG) by high-coverage single-cell RNA-sequencing (10 950 +/- 1 218 genes per neuron) and neuron size-based hierarchical clustering. Moreover, single DRG neurons responding to cutaneous stimuli are recorded using an in vivo whole-cell patch clamp technique and classified by neuron-type genetic markers. Small diameter DRG neurons are classified into one type of low-threshold mechanoreceptor and five types of mechanoheat nociceptors (MHNs). Each of the MHN types is further categorized into two subtypes. Large DRG neurons are categorized into four types, including neurexophilin 1-expressing MHNs and mechanical nociceptors (MNs) expressing BAI1-associated protein 2-like 1 (Baiap2l1). Mechanoreceptors expressing trafficking protein particle complex 3-like and Baiap2l1-marked MNs are subdivided into two subtypes each. These results provide a new system for cataloging somatosensory neurons and their transcriptome databases.
关键词	primary sensory neuron neuron type single-cell RNA sequencing somatosensation
收录类别	SCI
语种	英语
资助项目	Chinese Academy of Sciences[XDB01020300]
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000367615200009
出版者	INST BIOCHEMISTRY & CELL BIOLOGY
WOS关键词	SENSORY NEURONS ; NATRIURETIC PEPTIDE ; DORSAL-ROOT ; ION-CHANNEL ; EXPRESSION ; RECEPTOR ; PAIN ; SEQ ; GANGLION ; INVOLVEMENT
原始文献类型	Article
引用统计	被引频次：264[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2056
专题	生命科学与技术学院生命科学与技术学院_特聘教授组_鲍岚组生命科学与技术学院_特聘教授组_张旭组生命科学与技术学院_硕士生
通讯作者	Zhang, Xu
作者单位	1.Chinese Acad Sci, Shanghai Inst Biol Sci, CAS Ctr Excellence Brain Sci, Inst Neurosci, Shanghai 20031, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, CAS Ctr Excellence Brain Sci, State Key Lab Neurosci, Shanghai 20031, Peoples R China 3.Natl Engn Ctr Biochip Shanghai, Shanghai, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Cell Biol, Shanghai, Peoples R China 5.ShanghaiTec Univ, Sch Life Sci & Technol, Shanghai 200031, Peoples R China 6.Chinese Acad Sci, XuHui Cent Hosp, Shanghai Clin Ctr, Shanghai, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Li, Chang-Lin,Li, Kai-Cheng,Wu, Dan,et al. Somatosensory neuron types identified by high-coverage single-cell RNA-sequencing and functional heterogeneity[J]. CELL RESEARCH,2016,26(1):83-102.
APA	Li, Chang-Lin.,Li, Kai-Cheng.,Wu, Dan.,Chen, Yan.,Luo, Hao.,...&Zhang, Xu.(2016).Somatosensory neuron types identified by high-coverage single-cell RNA-sequencing and functional heterogeneity.CELL RESEARCH,26(1),83-102.
MLA	Li, Chang-Lin,et al."Somatosensory neuron types identified by high-coverage single-cell RNA-sequencing and functional heterogeneity".CELL RESEARCH 26.1(2016):83-102.