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Microfluidics for secretome analysis under enhanced endogenous signaling
2018-03-04
发表期刊BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (IF:2.5[JCR-2023],2.7[5-Year])
ISSN0006-291X
卷号497期号:2页码:480-484
发表状态已发表
DOI10.1016/j.bbrc.2018.02.025
摘要Cell secretome, the complex set of proteins that are secreted by the cells, is a fundamental mechanism of cell-cell communication both in vitro and in vivo. In vivo, the analysis of proteins secreted into body fluids can bring to the identification of biomarkers for important physiopathological conditions. However, due to the complexity of the protein content of body fluids, a better understanding of the secreted proteins by different cell types is highly desirable and can be performed in vitro for dissection. To this aim, microfluidic culture systems could be particularly relevant because of the accumulation of extrinsic endogenous signals at microliter scale, which better preserves the self-regulation occurring in the small interstitial spaces in vivo. In this work, we perform a quantitative study to compare the secretome in microfluidics and in a standard well plate. Human foreskin fibroblasts are used as a case study. This work also represents an important technological advance in terms of feasibility of high-throughput quantitative protein analyses in microfluidics. (C) 2018 Published by Elsevier Inc.
关键词Mass spectrometry Isotope label Microfluidic Secreted protein Conditioned medium Fibroblast
收录类别SCI ; SCIE
语种英语
资助项目Natural Science Foundation of China[31601178]
WOS研究方向Biochemistry & Molecular Biology ; Biophysics
WOS类目Biochemistry & Molecular Biology ; Biophysics
WOS记录号WOS:000428229400003
出版者ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS关键词PLURIPOTENT STEM-CELLS ; MASS-SPECTROMETRY ; PROTEINS
原始文献类型Article
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文献类型期刊论文
条目标识符https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/20246
专题生命科学与技术学院
免疫化学研究所_特聘教授组_生物工程学实验室
生命科学与技术学院_硕士生
通讯作者Elvassore, Nicola
作者单位
1.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, 393 Huaxia Rd, Shanghai 201210, Peoples R China
2.ShanghaiTech Univ, Sch Life Sci & Technol, 393 Huaxia Rd, Shanghai 201210, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China
4.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
5.Univ Padua, Dept Ind Engn, Via Marzolo 9, I-35131 Padua, Italy
6.Venetian Inst Mol Med, Via Orus 2, I-35129 Padua, Italy
第一作者单位免疫化学研究所;  生命科学与技术学院
通讯作者单位免疫化学研究所
第一作者的第一单位免疫化学研究所
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GB/T 7714
Hu, Qianjiang,Luni, Camilla,Elvassore, Nicola. Microfluidics for secretome analysis under enhanced endogenous signaling[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2018,497(2):480-484.
APA Hu, Qianjiang,Luni, Camilla,&Elvassore, Nicola.(2018).Microfluidics for secretome analysis under enhanced endogenous signaling.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,497(2),480-484.
MLA Hu, Qianjiang,et al."Microfluidics for secretome analysis under enhanced endogenous signaling".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 497.2(2018):480-484.
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