ShanghaiTech University Knowledge Management System
| De novo synthesis of 2'-fucosyllactose in engineered Bacillus subtilis ATCC 6051a | |
| 2022-09 | |
| 发表期刊 | PROCESS BIOCHEMISTRY (IF:3.7[JCR-2023],3.9[5-Year]) |
| ISSN | 1359-5113 |
| EISSN | 1873-3298 |
| 卷号 | 120页码:178-185 |
| 发表状态 | 已发表 |
| DOI | 10.1016/j.procbio.2022.06.007 |
| 摘要 | Microbial synthesis of 2'-fucosyllactose (2'-FL) has received much attention in recent years. In this study, Bacillus subtilis ATCC 6051a, a nonpathogenic GRAS (generally recognized as safe) organism, was engineered to produce 2'-FL. After a synthetic pathway comprising six genes was incorporated into the host cell genome, only a low level of 2'-FL (120 mg/L) was initially detected in recombinant cell culture. Comparison of heterologous lactose transporters confirmed the superior role of LacY from Escherichia coli for efficient production of 2'-FL in B. subtilis. There are two β-galactosidases, GanA and YesZ, in B. subtilis. Deletion of ganA and yesZ caused by insertion of the lacY cassette led to 2'-FL accumulation in shaking-flask culture at concentrations of 3.13 g/L and 5.56 g/L, respectively, which increased to 6.13 g/L in the double-deletion strain 164FL-GY. In addition, enhanced xylose metabolism in 164FL-GY further increased the concentration of 2'-FL to 7.14 g/L. In fed-batch fermentation, the highest productivity of 0.56 g/L·h was achieved in glycerol and xylose containing medium using engineered B. subtilis, and the accumulated 2'-FL reached 31.2 g/L. This is the first report of recombinant B. subtilis for high-level production of 2'-FL via a de novo synthesis pathway. © 2022 Elsevier Ltd |
| 关键词 | Bacteriology Cell culture Escherichia coli Metabolism Xylose 2'-fucosyllactose B.subtilis Bacillus Subtilis De novo synthesis Fucosyllactose GDP-ʟ -fucose Host cells Microbial synthesis Six genes Synthetic pathways |
| URL | 查看原文 |
| 收录类别 | EI ; SCI ; SCIE |
| 语种 | 英语 |
| 资助项目 | National Natural Science Foun-dation of China["32070055","51961125202"] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering |
| WOS类目 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical |
| WOS记录号 | WOS:000861725300001 |
| 出版者 | Elsevier Ltd |
| EI入藏号 | 20222512255726 |
| EI主题词 | Metabolic engineering |
| EI分类号 | 461.1 Biomedical Engineering ; 461.8.1 Genetic Engineering ; 804.1 Organic Compounds |
| 原始文献类型 | Journal article (JA) |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/200679 |
| 专题 | 生命科学与技术学院_硕士生 生命科学与技术学院_特聘教授组_孙俊松组 生命科学与技术学院_特聘教授组_史吉平组 |
| 通讯作者 | Shi, Jiping; Sun, Junsong |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Adv Res Inst, Shanghai 201210, Peoples R China 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 通讯作者单位 | 生命科学与技术学院 |
| 推荐引用方式 GB/T 7714 | Ji, Minghua,Liu, Yufei,Xie, Shiqiu,et al. De novo synthesis of 2'-fucosyllactose in engineered Bacillus subtilis ATCC 6051a[J]. PROCESS BIOCHEMISTRY,2022,120:178-185. |
| APA | Ji, Minghua.,Liu, Yufei.,Xie, Shiqiu.,Fu, Cong.,Liu, Min.,...&Sun, Junsong.(2022).De novo synthesis of 2'-fucosyllactose in engineered Bacillus subtilis ATCC 6051a.PROCESS BIOCHEMISTRY,120,178-185. |
| MLA | Ji, Minghua,et al."De novo synthesis of 2'-fucosyllactose in engineered Bacillus subtilis ATCC 6051a".PROCESS BIOCHEMISTRY 120(2022):178-185. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
修改评论
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。