Generation of an Oocyte-Specific Cas9 Transgenic Mouse for Genome Editing

doi:10.1371/journal.pone.0154364

	Generation of an Oocyte-Specific Cas9 Transgenic Mouse for Genome Editing
	Zhang, Linlin 1; Zhou, Jiankui 1; Han, Jinxiong 1; Hu, Bian 1; Hou, Ningning 1; Shi, Yun 1; Huang, Xingxu1,2 ; Lou, Xin 1
	2016-04-27
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	11 期号:4
发表状态	已发表
DOI	10.1371/journal.pone.0154364
摘要	The CRISPR/Cas9 system has been developed as an easy-handle and multiplexable approach for engineering eukaryotic genomes by zygote microinjection of Cas9 and sgRNA, while preparing Cas9 for microinjection is laborious and introducing inconsistency into the experiment. Here, we describe a modified strategy for gene targeting through using oocyte-specific Cas9 transgenic mouse. With this mouse line, we successfully achieve precise gene targeting by injection of sgRNAs only into one-cell-stage embryos. Through comprehensive analysis, we also show allele complexity and off-target mutagenesis induced by this strategy is obviously lower than Cas9 mRNA/sgRNA injection. Thus, injection of sgRNAs into oocyte-specific Cas9 transgenic mouse embryo provides a convenient, efficient and reliable approach for mouse genome editing.
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[NSFC 31471354] ; National Natural Science Foundation of China[NSFC 31471400]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000374976200063
出版者	PUBLIC LIBRARY SCIENCE
WOS关键词	MODIFIED MICE ; DNA ; CRISPR-CAS9 ; SYSTEM ; NUCLEASES
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1862
专题	生命科学与技术学院_PI研究组_黄行许组
通讯作者	Huang, Xingxu; Lou, Xin
作者单位	1.Nanjing Univ, MOE Key Lab Model Anim Dis Study, Model Anim Res Ctr, Nanjing 210008, Jiangsu, Peoples R China 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Zhang, Linlin,Zhou, Jiankui,Han, Jinxiong,et al. Generation of an Oocyte-Specific Cas9 Transgenic Mouse for Genome Editing[J]. PLOS ONE,2016,11(4).
APA	Zhang, Linlin.,Zhou, Jiankui.,Han, Jinxiong.,Hu, Bian.,Hou, Ningning.,...&Lou, Xin.(2016).Generation of an Oocyte-Specific Cas9 Transgenic Mouse for Genome Editing.PLOS ONE,11(4).
MLA	Zhang, Linlin,et al."Generation of an Oocyte-Specific Cas9 Transgenic Mouse for Genome Editing".PLOS ONE 11.4(2016).