Generation of GHR-modified pigs as Laron syndrome models via a dual-sgRNAs/Cas9 system and somatic cell nuclear transfer

doi:10.1186/s12967-018-1409-7

	Generation of GHR-modified pigs as Laron syndrome models via a dual-sgRNAs/Cas9 system and somatic cell nuclear transfer
	Yu, Honghao 1,5; Long, Weihu 2,3; Zhang, Xuezeng 2,3; Xu, Kaixiang 2; Guo, Jianxiong 2,3; Zhao, Heng 2,3; Li, Honghui 2,3; Qing, Yubo 2,3; Pan, Weirong 3; Jia, Baoyu 2,3; Zhao, Hong-Ye 2; Huang, Xingxu1 ; Wei, Hong-Jiang 2,4
	2018-02-27
发表期刊	JOURNAL OF TRANSLATIONAL MEDICINE
ISSN	1479-5876
卷号	16
发表状态	已发表
DOI	10.1186/s12967-018-1409-7
摘要	Background: Laron syndrome is an autosomal disease resulting from mutations in the growth hormone receptor (GHR) gene. The only therapeutic treatment for Laron syndrome is recombinant insulin-like growth factor I (IGF-I), which has been shown to have various side effects. The improved Laron syndrome models are important for better understanding the pathogenesis of the disease and developing corresponding therapeutics. Pigs have become attractive biomedical models for human condition due to similarities in anatomy, physiology, and metabolism relative to humans, which could serve as an appropriate model for Laron syndrome.
关键词	GHR knockout Dual-sgRNAs/Cas9 Laron syndrome SCNT Diannan miniature pig
收录类别	SCI ; SCIE
语种	英语
资助项目	Innovative Research Team of Science and Technology in Yunnan Province, Innovative Research Team (in Science and Technology) in University of Yunnan Province and Major Program on Basic Research Projects of Yunnan Province[2014FC006]
WOS研究方向	Research & Experimental Medicine
WOS类目	Medicine, Research & Experimental
WOS记录号	WOS:000426717600001
出版者	BIOMED CENTRAL LTD
WOS关键词	GROWTH-HORMONE RECEPTOR ; INTRACELLULAR DOMAIN ; CRISPR/CAS9 SYSTEM ; GENE DELETION ; MUTATION ; PROTEIN ; MOUSE ; GENOME ; SWINE ; MICE
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/18293
专题	生命科学与技术学院_PI研究组_黄行许组
通讯作者	Zhao, Hong-Ye; Huang, Xingxu; Wei, Hong-Jiang
作者单位	1.ShanghaiTech Univ, Sch Life Sci & Technol, 100 Haike Rd, Shanghai 201210, Peoples R China 2.Yunnan Agr Univ, State Key Lab Conservat & Utilizat Bio Resources, Kunming 650201, Yunnan, Peoples R China 3.Yunnan Agr Univ, Coll Anim Sci & Technol, Kunming 650201, Yunnan, Peoples R China 4.Yunnan Agr Univ, Coll Vet Med, Kunming 650201, Yunnan, Peoples R China 5.Guilin Med Univ, Coll Biotechnol, Guilin 541100, Peoples R China
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
第一作者的第一单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Yu, Honghao,Long, Weihu,Zhang, Xuezeng,et al. Generation of GHR-modified pigs as Laron syndrome models via a dual-sgRNAs/Cas9 system and somatic cell nuclear transfer[J]. JOURNAL OF TRANSLATIONAL MEDICINE,2018,16.
APA	Yu, Honghao.,Long, Weihu.,Zhang, Xuezeng.,Xu, Kaixiang.,Guo, Jianxiong.,...&Wei, Hong-Jiang.(2018).Generation of GHR-modified pigs as Laron syndrome models via a dual-sgRNAs/Cas9 system and somatic cell nuclear transfer.JOURNAL OF TRANSLATIONAL MEDICINE,16.
MLA	Yu, Honghao,et al."Generation of GHR-modified pigs as Laron syndrome models via a dual-sgRNAs/Cas9 system and somatic cell nuclear transfer".JOURNAL OF TRANSLATIONAL MEDICINE 16(2018).