Molecular basis for cell-wall recycling regulation by transcriptional repressor MurR in Escherichia coli
2022-06-10
发表期刊NUCLEIC ACIDS RESEARCH (IF:16.6[JCR-2023],16.1[5-Year])
ISSN0305-1048
EISSN1362-4962
DOI10.1093/nar/gkac442
摘要

The cell-wall recycling process is important for bacterial survival in nutrient-limited conditions and, in certain cases, is directly involved in antibiotic resistance. In the sophisticated cell-wall recycling process in Escherichia coli, the transcriptional repressor MurR controls the expression of murP and murQ, which are involved in transporting and metabolizing N-acetylmuramic acid (MurNAc), generating N-acetylmuramic acid-6-phosphate (MurNAc-6-P) and N-acetylglucosamine-6-phosphate (GlcNAc-6-P). Here, we report that both MurNAc-6-P and GlcNAc-6-P can bind to MurR and weaken the DNA binding ability of MurR. Structural characterizations of MurR in complex with MurNAc-6-P or GlcNAc-6-P as well as in the apo form revealed the detailed ligand recognition chemistries. Further studies showed that only MurNAc-6-P, but not GlcNAc-6-P, is capable of derepressing the expression of murQP controlled by MurR in cells and clarified the substrate specificity through the identification of key residues responsible for ligand binding in the complex structures. In summary, this study deciphered the molecular mechanism of the cell wall recycling process regulated by MurR in E. coli.

关键词Escherichia coli cell-wall recycling molecular mechanism
学科门类理学
收录类别SCI ; SCIE
资助项目National Natural Science Foundation of China[91753127,21922705,22077083,21907066] ; Lingang Laboratory[LG-QS-202206-05] ; Shanghai Science and Technology Committee["22ZR1480100","19JC1410300"]
WOS研究方向Biochemistry & Molecular Biology
WOS类目Biochemistry & Molecular Biology
WOS记录号WOS:000805252500001
引用统计
正在获取...
文献类型期刊论文
条目标识符https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/178420
专题物质科学与技术学院_PI研究组_李健组
物质科学与技术学院_PI研究组_季泉江组
物质科学与技术学院_博士生
共同第一作者Weizhong, Chen; Di, Wu
通讯作者Shu-Yu, Zhang; Quanjiang, Ji
作者单位
1.ShanghaiTech University, School of Physical Science and Technology
2.University of Chinese Academy of Sciences
3.Shanghai Jiao Tong University, School of Chemistry and Chemical Engineering & Frontiers Science Center for Transformative Molecules
第一作者单位物质科学与技术学院
通讯作者单位物质科学与技术学院
第一作者的第一单位物质科学与技术学院
推荐引用方式
GB/T 7714
Ya, Zhang,Weizhong, Chen,Di, Wu,et al. Molecular basis for cell-wall recycling regulation by transcriptional repressor MurR in Escherichia coli[J]. NUCLEIC ACIDS RESEARCH,2022.
APA Ya, Zhang.,Weizhong, Chen.,Di, Wu.,Yushi, Liu.,Zhaowei, Wu.,...&Quanjiang, Ji.(2022).Molecular basis for cell-wall recycling regulation by transcriptional repressor MurR in Escherichia coli.NUCLEIC ACIDS RESEARCH.
MLA Ya, Zhang,et al."Molecular basis for cell-wall recycling regulation by transcriptional repressor MurR in Escherichia coli".NUCLEIC ACIDS RESEARCH (2022).
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
个性服务
查看访问统计
谷歌学术
谷歌学术中相似的文章
[Ya, Zhang]的文章
[Weizhong, Chen]的文章
[Di, Wu]的文章
百度学术
百度学术中相似的文章
[Ya, Zhang]的文章
[Weizhong, Chen]的文章
[Di, Wu]的文章
必应学术
必应学术中相似的文章
[Ya, Zhang]的文章
[Weizhong, Chen]的文章
[Di, Wu]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。