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Molecular basis for cell-wall recycling regulation by transcriptional repressor MurR in Escherichia coli | |
2022-06-10 | |
发表期刊 | NUCLEIC ACIDS RESEARCH (IF:16.6[JCR-2023],16.1[5-Year]) |
ISSN | 0305-1048 |
EISSN | 1362-4962 |
DOI | 10.1093/nar/gkac442 |
摘要 | The cell-wall recycling process is important for bacterial survival in nutrient-limited conditions and, in certain cases, is directly involved in antibiotic resistance. In the sophisticated cell-wall recycling process in Escherichia coli, the transcriptional repressor MurR controls the expression of murP and murQ, which are involved in transporting and metabolizing N-acetylmuramic acid (MurNAc), generating N-acetylmuramic acid-6-phosphate (MurNAc-6-P) and N-acetylglucosamine-6-phosphate (GlcNAc-6-P). Here, we report that both MurNAc-6-P and GlcNAc-6-P can bind to MurR and weaken the DNA binding ability of MurR. Structural characterizations of MurR in complex with MurNAc-6-P or GlcNAc-6-P as well as in the apo form revealed the detailed ligand recognition chemistries. Further studies showed that only MurNAc-6-P, but not GlcNAc-6-P, is capable of derepressing the expression of murQP controlled by MurR in cells and clarified the substrate specificity through the identification of key residues responsible for ligand binding in the complex structures. In summary, this study deciphered the molecular mechanism of the cell wall recycling process regulated by MurR in E. coli. |
关键词 | Escherichia coli cell-wall recycling molecular mechanism |
学科门类 | 理学 |
收录类别 | SCI ; SCIE |
资助项目 | National Natural Science Foundation of China[91753127,21922705,22077083,21907066] ; Lingang Laboratory[LG-QS-202206-05] ; Shanghai Science and Technology Committee["22ZR1480100","19JC1410300"] |
WOS研究方向 | Biochemistry & Molecular Biology |
WOS类目 | Biochemistry & Molecular Biology |
WOS记录号 | WOS:000805252500001 |
引用统计 | 正在获取...
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文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/178420 |
专题 | 物质科学与技术学院_PI研究组_李健组 物质科学与技术学院_PI研究组_季泉江组 物质科学与技术学院_博士生 |
共同第一作者 | Weizhong, Chen; Di, Wu |
通讯作者 | Shu-Yu, Zhang; Quanjiang, Ji |
作者单位 | 1.ShanghaiTech University, School of Physical Science and Technology 2.University of Chinese Academy of Sciences 3.Shanghai Jiao Tong University, School of Chemistry and Chemical Engineering & Frontiers Science Center for Transformative Molecules |
第一作者单位 | 物质科学与技术学院 |
通讯作者单位 | 物质科学与技术学院 |
第一作者的第一单位 | 物质科学与技术学院 |
推荐引用方式 GB/T 7714 | Ya, Zhang,Weizhong, Chen,Di, Wu,et al. Molecular basis for cell-wall recycling regulation by transcriptional repressor MurR in Escherichia coli[J]. NUCLEIC ACIDS RESEARCH,2022. |
APA | Ya, Zhang.,Weizhong, Chen.,Di, Wu.,Yushi, Liu.,Zhaowei, Wu.,...&Quanjiang, Ji.(2022).Molecular basis for cell-wall recycling regulation by transcriptional repressor MurR in Escherichia coli.NUCLEIC ACIDS RESEARCH. |
MLA | Ya, Zhang,et al."Molecular basis for cell-wall recycling regulation by transcriptional repressor MurR in Escherichia coli".NUCLEIC ACIDS RESEARCH (2022). |
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