Single-pulse enhanced coherent diffraction imaging of bacteria with an X-ray free-electron laser

doi:10.1038/srep34008

KMS > iHuman研究所

	Single-pulse enhanced coherent diffraction imaging of bacteria with an X-ray free-electron laser
	Fan, Jiadong 1; Sun, Zhibin 1; Wang, Yaling 2; Park, Jaehyun 3; Kim, Sunam 3; Gallagher-Jones, Marcus 4,5; Kim, Yoonhee 6; Song, Changyong 7; Yao, Shengkun 1; Zhang, Jian 1; Zhang, Jianhua 1; Duan, Xiulan 1; Tono, Kensuke 8; Yabashi, Makina 3; Ishikawa, Tetsuya 3; Fan, Chunhai 9; Zhao, Yuliang 2; Chai, Zhifang 2; Gao, Xueyun 2; Earnest, Thomas9,10 ; Jiang, Huaidong1,11
	2016-09-23
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	6
发表状态	已发表
DOI	10.1038/srep34008
摘要	High-resolution imaging offers one of the most promising approaches for exploring and understanding the structure and function of biomaterials and biological systems. X-ray free-electron lasers (XFELs) combined with coherent diffraction imaging can theoretically provide high-resolution spatial information regarding biological materials using a single XFEL pulse. Currently, the application of this method suffers from the low scattering cross-section of biomaterials and X-ray damage to the sample. However, XFELs can provide pulses of such short duration that the data can be collected using the "diffract and destroy" approach before the effects of radiation damage on the data become significant. These experiments combine the use of enhanced coherent diffraction imaging with single-shot XFEL radiation to investigate the cellular architecture of Staphylococcus aureus with and without labeling by gold (Au) nanoclusters. The resolution of the images reconstructed from these diffraction patterns were twice as high or more for gold-labeled samples, demonstrating that this enhancement method provides a promising approach for the high-resolution imaging of biomaterials and biological systems.
收录类别	SCI
语种	英语
资助项目	NRF[2015R1A5A1009962] ; NRF[2016R1A2B3010980]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000384045500001
出版者	NATURE PUBLISHING GROUP
WOS关键词	MAGNETOTACTIC BACTERIA ; GOLD NANOPARTICLES ; ATOMIC-RESOLUTION ; MICROSCOPY ; CELLS ; RECONSTRUCTION ; CRYSTALLOGRAPHY ; MECHANISMS ; SCATTERING ; NOISE
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1704
专题	iHuman研究所物质科学与技术学院大科学中心_PI研究组_江怀东组
通讯作者	Gao, Xueyun; Jiang, Huaidong
作者单位	1.Shandong Univ, State Key Lab Crystal Mat, Jinan 250100, Peoples R China 2.Chinese Acad Sci, Inst High Energy Phys, Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100049, Peoples R China 3.RIKEN SPring 8 Ctr, Kouto 1-1-1, Sayo, Hyogo 6795148, Japan 4.Univ Calif Los Angeles, Dept Phys & Astron, Los Angeles, CA 90095 USA 5.Univ Calif Los Angeles, Calif NanoSyst Inst, Los Angeles, CA 90095 USA 6.Gwangju Inst Sci & Technol, Sch Mat Sci & Engn, Gwangju 61005, South Korea 7.POSTECH, Dept Phys, Pohang 790784, South Korea 8.Japan Synchrotron Radiat Res Inst JASRI, 1-1-1 Kouto, Sayo, Hyogo 6795198, Japan 9.Acad Sinica, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Shanghai 201800, Peoples R China 10.Shanghai Tech Univ, iHuman Inst, Shanghai 201210, Peoples R China 11.Shanghai Tech Univ, Sch Phys Sci & Technol, Shanghai 201210, Peoples R China
通讯作者单位	物质科学与技术学院
推荐引用方式 GB/T 7714	Fan, Jiadong,Sun, Zhibin,Wang, Yaling,et al. Single-pulse enhanced coherent diffraction imaging of bacteria with an X-ray free-electron laser[J]. SCIENTIFIC REPORTS,2016,6.
APA	Fan, Jiadong.,Sun, Zhibin.,Wang, Yaling.,Park, Jaehyun.,Kim, Sunam.,...&Jiang, Huaidong.(2016).Single-pulse enhanced coherent diffraction imaging of bacteria with an X-ray free-electron laser.SCIENTIFIC REPORTS,6.
MLA	Fan, Jiadong,et al."Single-pulse enhanced coherent diffraction imaging of bacteria with an X-ray free-electron laser".SCIENTIFIC REPORTS 6(2016).