Dynamic chromatin state profiling reveals regulatory roles of auxin and cytokinin in shoot regeneration

doi:10.1016/j.devcel.2021.12.019

	Dynamic chromatin state profiling reveals regulatory roles of auxin and cytokinin in shoot regeneration
	Wu, Lian-Yu1,2,3 ; Shang, Guan-Dong 1,3; Wang, Fu-Xiang 1,3; Gao, Jian 1,3; Wan, Mu-Chun1,2 ; Xu, Zhou-Geng 1,3; Wang, Jia-Wei1,2
	2022-02-28
发表期刊	DEVELOPMENTAL CELL (IF:10.7[JCR-2023],11.5[5-Year])
ISSN	1534-5807
EISSN	1878-1551
卷号	57 期号:4
发表状态	已发表
DOI	10.1016/j.devcel.2021.12.019
摘要	Shoot regeneration is mediated by the sequential action of two phytohormones, auxin and cytokinin. However, the chromatin regulatory landscapes underlying this dynamic response have not yet been studied. In this study, we jointly profiled chromatin accessibility, histone modifications, and transcriptomes to demonstrate that a high auxin/cytokinin ratio environment primes Arabidopsis shoot regeneration by increasing the accessibility of the gene loci associated with pluripotency and shoot fate determination. Cytokinin signaling not only triggers the commitment of the shoot progenitor at later stages but also allows chromatin to maintain shoot identity genes at the priming stage. Our analysis of transcriptional regulatory dynamics further identifies a catalog of regeneration cis-elements dedicated to cell fate transitions and uncovers important roles of BES1, MYC, IDD, and PIF transcription factors in shoot regeneration. Our results, thus, provide a comprehensive resource for studying cell reprogramming in plants and provide potential targets for improving future shoot regeneration efficiency.
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收录类别	SCI ; SCIE
语种	英语
资助项目	National Natural Science Foundation of China[31788103,31721001] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB27030101]
WOS研究方向	Cell Biology ; Developmental Biology
WOS类目	Cell Biology ; Developmental Biology
WOS记录号	WOS:000767291200001
出版者	CELL PRESS
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/165007
专题	生命科学与技术学院_博士生生命科学与技术学院_特聘教授组_王佳伟组
通讯作者	Wang, Jia-Wei
作者单位	1.Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, Inst Plant Physiol & Ecol SIPPE, Natl Key Lab Plant Mol Genet NKLPMG, Shanghai 200032, Peoples R China 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 3.Univ Chinese Acad Sci UCAS, Shanghai 200032, Peoples R China
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Wu, Lian-Yu,Shang, Guan-Dong,Wang, Fu-Xiang,et al. Dynamic chromatin state profiling reveals regulatory roles of auxin and cytokinin in shoot regeneration[J]. DEVELOPMENTAL CELL,2022,57(4).
APA	Wu, Lian-Yu.,Shang, Guan-Dong.,Wang, Fu-Xiang.,Gao, Jian.,Wan, Mu-Chun.,...&Wang, Jia-Wei.(2022).Dynamic chromatin state profiling reveals regulatory roles of auxin and cytokinin in shoot regeneration.DEVELOPMENTAL CELL,57(4).
MLA	Wu, Lian-Yu,et al."Dynamic chromatin state profiling reveals regulatory roles of auxin and cytokinin in shoot regeneration".DEVELOPMENTAL CELL 57.4(2022).