Genome-Editing Technologies: Principles and Applications

doi:10.1101/cshperspect.a023754

	Genome-Editing Technologies: Principles and Applications
	Gaj, Thomas 1; Sirk, Shannon J.2; Shui, Sai-lan3 ; Liu, Jia3
	2016-12
发表期刊	COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY
ISSN	1943-0264
卷号	8 期号:12
发表状态	已发表
DOI	10.1101/cshperspect.a023754
摘要	Targeted nucleases have provided researchers with the ability to manipulate virtually any genomic sequence, enabling the facile creation of isogenic cell lines and animal models for the study of human disease, and promoting exciting new possibilities for human gene therapy. Here we review three foundational technologies-clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9), transcription activator-like effector nucleases (TALENs), and zinc-finger nucleases (ZFNs). We discuss the engineering advances that facilitated their development and highlight several achievements in genome engineering that were made possible by these tools. We also consider artificial transcription factors, illustrating how this technology can complement targeted nucleases for synthetic biology and gene therapy.
收录类别	SCI
语种	英语
资助项目	National Institutes of Health[F32GM113446]
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000390367600003
出版者	COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
WOS关键词	ZINC-FINGER NUCLEASES ; ARTIFICIAL TRANSCRIPTION FACTORS ; CONTROLLING GENE-EXPRESSION ; HOMOLOGY-DIRECTED REPAIR ; RNA-GUIDED ENDONUCLEASE ; DNA-BINDING SPECIFICITY ; TAL EFFECTOR NUCLEASES ; DOUBLE-STRAND BREAKS ; CRISPR-CAS SYSTEMS ; IN-VITRO SELECTION
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1624
专题	生命科学与技术学院免疫化学研究所_特聘教授组_抗体化学实验室生命科学与技术学院_硕士生免疫化学研究所_PI研究组_刘佳组
通讯作者	Gaj, Thomas; Liu, Jia
作者单位	1.Univ Calif Berkeley, Dept Bioengn, Berkeley, CA 94720 USA 2.Stanford Univ, Dept Chem Engn, Stanford, CA 94305 USA 3.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai, Peoples R China
通讯作者单位	免疫化学研究所
推荐引用方式 GB/T 7714	Gaj, Thomas,Sirk, Shannon J.,Shui, Sai-lan,et al. Genome-Editing Technologies: Principles and Applications[J]. COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY,2016,8(12).
APA	Gaj, Thomas,Sirk, Shannon J.,Shui, Sai-lan,&Liu, Jia.(2016).Genome-Editing Technologies: Principles and Applications.COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY,8(12).
MLA	Gaj, Thomas,et al."Genome-Editing Technologies: Principles and Applications".COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY 8.12(2016).