MicroRNA Expression Profile Analysis of Chlamydomonas reinhardtii during Lipid Accumulation Process under Nitrogen Deprivation Stresses

doi:10.3390/bioengineering9010006

	MicroRNA Expression Profile Analysis of Chlamydomonas reinhardtii during Lipid Accumulation Process under Nitrogen Deprivation Stresses
	Zhang, Jingxian 1,2; Shi, Jiping1,2,3 ; Yuan, Chenyang1,2,3 ; Liu, Xiangcen 1,2; Du, Guilin1,2,3 ; Fan, Ruimei 4,5; Zhang, Baoguo 1,2
	2022-01
发表期刊	BIOENGINEERING-BASEL (IF:3.8[JCR-2023])
EISSN	2306-5354
卷号	9 期号:1
发表状态	已发表
DOI	10.3390/bioengineering9010006
摘要	Lipid accumulation in various microalgae has been found induced by nitrogen deprivation, and it controls many different genes expression. Yet, the underlying molecular mechanisms still remain largely unknown. MicroRNA (miRNAs) play a critical role in post-transcriptional gene regulation. In this study, miRNAs were hypothesized involved in lipid accumulation by nitrogen deprivation. A deep-sequencing platform was used to explore miRNAs-mediated responses induced by nitrogen deprivation in Chlamydomonas reinhardtii. The eukaryotic orthologous groups of proteins (KOG) function in the predicted target genes of miRNA with response to nitrogen deprivation were mainly involved in signal transduction mechanisms, including transcription, lipid transport, and metabolism. A total of 109 miRNA were predicted, including 79 known miRNA and 30 novel miRNA. A total of 29 miRNAs showed significantly differential expressions after nitrogen deprivation, and most of them were upregulated. A total of 10 miRNAs and their targeting genes might involve in lipid transport and metabolism biological process. This study first investigates nitrogen deprivation-regulated miRNAs in microalgae and broadens perspectives on miRNAs importance in microalgae lipid accumulation via nitrogen deprivation. This study provides theoretical guidance for the application of microalgae in bio-oil engineering production.
关键词	microRNA Chlamydomonas reinhardtii lipid nitrogen deprivation
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收录类别	SCI ; SCIE
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology ; Engineering
WOS类目	Biotechnology & Applied Microbiology ; Engineering, Biomedical
WOS记录号	WOS:000758429100001
出版者	MDPI
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/159558
专题	生命科学与技术学院_博士生生命科学与技术学院_特聘教授组_史吉平组
通讯作者	Fan, Ruimei; Zhang, Baoguo
作者单位	1.Chinese Acad Sci, Shanghai Adv Res Inst, Lab Biorefinery, 99 Haike Rd, Shanghai 201210, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 4.Xinxiang Med Univ, Sino UK Joint Lab Brain Funct & Injury, Xinxiang 453003, Henan, Peoples R China 5.Xinxiang Med Univ, Dept Physiol & Neurobiol, Xinxiang 453003, Henan, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Jingxian,Shi, Jiping,Yuan, Chenyang,et al. MicroRNA Expression Profile Analysis of Chlamydomonas reinhardtii during Lipid Accumulation Process under Nitrogen Deprivation Stresses[J]. BIOENGINEERING-BASEL,2022,9(1).
APA	Zhang, Jingxian.,Shi, Jiping.,Yuan, Chenyang.,Liu, Xiangcen.,Du, Guilin.,...&Zhang, Baoguo.(2022).MicroRNA Expression Profile Analysis of Chlamydomonas reinhardtii during Lipid Accumulation Process under Nitrogen Deprivation Stresses.BIOENGINEERING-BASEL,9(1).
MLA	Zhang, Jingxian,et al."MicroRNA Expression Profile Analysis of Chlamydomonas reinhardtii during Lipid Accumulation Process under Nitrogen Deprivation Stresses".BIOENGINEERING-BASEL 9.1(2022).