Opposing Roles of Acetylation and Phosphorylation in LIFR-Dependent Self-Renewal Growth Signaling in Mouse Embryonic Stem Cells

doi:10.1016/j.celrep.2016.12.081

	Opposing Roles of Acetylation and Phosphorylation in LIFR-Dependent Self-Renewal Growth Signaling in Mouse Embryonic Stem Cells
	Wang, Xiong-jun 1,2; Qiao, Yunbo3,4 ; Xiao, Minzhe M.1; Wang, Lingbo 3; Chen, Jun 3,5; Lv, Wenjian 3; Xu, Li 6; Li, Yan 6; Wang, Yumei 1; Tan, Ming-dian 1; Huang, Chao 1; Li, Jinsong 3; Zhao, Ting C.7; Hou, Zhaoyuan 2; Jing, Naihe 3; Chin, Y. Eugene 1
	2017-01-24
发表期刊	CELL REPORTS (IF:7.5[JCR-2023],8.5[5-Year])
ISSN	2211-1247
卷号	18 期号:4 页码:933-946
发表状态	已发表
DOI	10.1016/j.celrep.2016.12.081
摘要	LIF promotes self-renewal of mouse embryonic stem cells (mESCs), and in its absence, the cells differentiate. LIF binds to the LIF receptor (LIFR) and activates the JAK-STAT3 pathway, but it remains unknown how the receptor complex triggers differentiation or self-renewal. Here, we report that the LIFR cytoplasmic domain contains a self-renewal domain within the juxtamembrane region and a differentiation domain within the C-terminal region. The differentiation domain contains four SPXX repeats that are phosphorylated by MAPK to restrict STAT3 activation; the self-renewal domain is characterized by a 3K motif that is acetylated by p300. In mESCs, acetyl-LIFR undergoes homodimerization, leading to STAT3 hypo- or hyper-activation depending on the presence or absence of gp130. LIFR-activated STAT3 restricts differentiation via cytokine induction. Thus, LIFR acetylation and serine phosphorylation differentially promote stem cell self-renewal and differentiation.
收录类别	SCI
语种	英语
资助项目	Chinese Academy of Science starting grant[2011CSP002]
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000396474300010
出版者	CELL PRESS
WOS关键词	LEUKEMIA INHIBITORY FACTOR ; PHASE RESPONSE FACTOR/STAT3 ; PROTEIN-KINASE ; GROUND-STATE ; DIFFERENTIAL ACTIVATION ; CYTOPLASMIC DOMAINS ; CYTOKINE RECEPTORS ; TRANSDUCER GP130 ; SINGLE CELLS ; PLURIPOTENCY
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1530
专题	iHuman研究所_PI研究组_钟桂生组
通讯作者	Hou, Zhaoyuan; Jing, Naihe; Chin, Y. Eugene
作者单位	1.Shanghai Jiao Tong Univ, Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Stem Cell Biol,Inst Hlth Sci,Sch Med, 320 Yueyang Rd, Shanghai 200031, Peoples R China 2.Shanghai Jiao Tong Univ, Hongqiao Inst Med, Sch Med, Shanghai Tongren Hosp,Fac Basic Med, Shanghai, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Cell Biol, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China 4.Shanghai Tech Univ, IHuman Inst, 99 Haike Rd, Shanghai 201210, Peoples R China 5.Nankai Univ, Dept Genet & Cell Biol, Coll Life Sci, Tianjin 300071, Peoples R China 6.Zhejiang Chinese Med Univ, Sch Basic Med, Dept Signal Transduct, Hangzhou 310053, Zhejiang, Peoples R China 7.Boston Univ, Dept Surg, Roger Williams Med Ctr, Sch Med, Providence, RI 02908 USA
推荐引用方式 GB/T 7714	Wang, Xiong-jun,Qiao, Yunbo,Xiao, Minzhe M.,et al. Opposing Roles of Acetylation and Phosphorylation in LIFR-Dependent Self-Renewal Growth Signaling in Mouse Embryonic Stem Cells[J]. CELL REPORTS,2017,18(4):933-946.
APA	Wang, Xiong-jun.,Qiao, Yunbo.,Xiao, Minzhe M..,Wang, Lingbo.,Chen, Jun.,...&Chin, Y. Eugene.(2017).Opposing Roles of Acetylation and Phosphorylation in LIFR-Dependent Self-Renewal Growth Signaling in Mouse Embryonic Stem Cells.CELL REPORTS,18(4),933-946.
MLA	Wang, Xiong-jun,et al."Opposing Roles of Acetylation and Phosphorylation in LIFR-Dependent Self-Renewal Growth Signaling in Mouse Embryonic Stem Cells".CELL REPORTS 18.4(2017):933-946.