Knockout of circRNAs by base editing back-splice sites of circularized exons

doi:10.1186/s13059-021-02563-0

	Knockout of circRNAs by base editing back-splice sites of circularized exons
	Gao, Xiang1,2 ; Ma, Xu Kai 3; Li, Xiang 1,4; Li, Guo Wei 3; Liu, Chu Xiao 1; Zhang, Jun 1; Wang, Ying 1; Wei, Jia 3; Chen, Jia2,5 ; Chen, Ling Ling 1,4; Yang, Li 3
	2022-01-10
发表期刊	GENOME BIOLOGY
ISSN	1474-760X
EISSN	1474-760X
卷号	23 期号:1
发表状态	已发表
DOI	10.1186/s13059-021-02563-0
摘要	Many circular RNAs (circRNAs) are produced from back-splicing of exons of precursor mRNAs and are generally co-expressed with cognate linear RNAs. Methods for circRNA-specific knockout are lacking, largely due to sequence overlaps between forms. Here, we use base editors (BEs) for circRNA depletion. By targeting splice sites involved in both back-splicing and canonical splicing, BEs can repress circular and linear RNAs. Targeting sites predominantly for circRNA biogenesis, BEs could efficiently repress the production of circular but not linear RNAs. As hundreds of exons are predominantly back-spliced to produce circRNAs, this provides an efficient method to deplete circRNAs for functional study.
关键词	Circular RNA Base editor Predominantly back-spliced exon Splicing Back-splicing Knockout
URL	查看原文
收录类别	SCI ; SCIE
语种	英语
资助项目	Ministry of Science and Technology of China, China[
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS类目	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS记录号	WOS:000740975300001
出版者	BMC
Scopus 记录号	2-s2.0-85122732515
来源库	Scopus
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/148737
专题	生命科学与技术学院_PI研究组_陈佳组生命科学与技术学院_博士生
通讯作者	Yang, Li
作者单位	1.State Key Laboratory of Molecular Biology,Shanghai Key Laboratory of Molecular Andrology,CAS Center for Excellence in Molecular Cell Science,Shanghai Institute of Biochemistry and Cell Biology,University of Chinese Academy of Sciences,Chinese Academy of Sciences,Shanghai,320 Yueyang Road,200031,China 2.School of Life Science and Technology,ShanghaiTech University,Shanghai,393 Middle Huaxia Road,201210,China 3.CAS Key Laboratory of Computational Biology,Shanghai Institute of Nutrition and Health,University of Chinese Academy of Sciences,Chinese Academy of Sciences,Shanghai,320 Yueyang Road,200031,China 4.Hangzhou Institute for Advanced Study,University of Chinese Academy of Sciences,Hangzhou,330106,China 5.CAS Center for Excellence in Molecular Cell Science,Shanghai Institute of Biochemistry and Cell Biology,Chinese Academy of Sciences,Shanghai,200031,China
第一作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Gao, Xiang,Ma, Xu Kai,Li, Xiang,et al. Knockout of circRNAs by base editing back-splice sites of circularized exons[J]. GENOME BIOLOGY,2022,23(1).
APA	Gao, Xiang.,Ma, Xu Kai.,Li, Xiang.,Li, Guo Wei.,Liu, Chu Xiao.,...&Yang, Li.(2022).Knockout of circRNAs by base editing back-splice sites of circularized exons.GENOME BIOLOGY,23(1).
MLA	Gao, Xiang,et al."Knockout of circRNAs by base editing back-splice sites of circularized exons".GENOME BIOLOGY 23.1(2022).