Two NPF transporters mediate iron long-distance transport and homeostasis in Arabidopsis

doi:10.1016/j.xplc.2021.100244

	Two NPF transporters mediate iron long-distance transport and homeostasis in Arabidopsis
	Chen, Si-Ying 1,2; Gu, Tian-Yu1,3 ; Qi, Zi-Ai 1,2; Yan, Jing 1,4; Fang, Zi-Jun 1; Lu, Yu-Ting 1,2; Li, Hui 1; Gong, Ji-Ming1,2,3
	2021-11-08
发表期刊	PLANT COMMUNICATIONS (IF:9.4[JCR-2023],9.4[5-Year])
ISSN	2590-3462
卷号	2 期号:6
DOI	10.1016/j.xplc.2021.100244
摘要	Iron (Fe) transport and reallocation are essential to Fe homeostasis in plants, but it is unclear how Fe homeostasis is regulated, especially under stress. Here we report that NPF5.9 and its close homolog NPF5.8 redundantly regulate Fe transport and reallocation in Arabidopsis. NPF5.9 is highly upregulated in response to Fe deficiency. NPF5.9 expresses preferentially in vasculature tissues and localizes to the trans-Golgi network, and NPF5.8 showed a similar expression pattern. Long-distance Fe transport and allocation into aerial parts was significantly increased in NPF5.9-overexpressing lines. In the double mutant npf5.8 npf5.9, Fe loading in aerial parts and plant growth were decreased, which were partially rescued by Fe supplementation. Further analysis showed that expression of PYE, the negative regulator for Fe homeostasis, and its downstream target NAS4 were significantly altered in the double mutant. NPF5.9 and NPF5.8 were shown to also mediate nitrate uptake and transport, although nitrate and Fe application did not reciprocally affect each other. Our findings uncovered the novel function of NPF5.9 and NPF5.8 in long-distance Fe transport and homeostasis, and further indicated that they possibly mediate nitrate transport and Fe homeostasis independently in Arabidopsis.
关键词	NPF transporter iron homeostasis nitrate transport and allocation trans-Golgi network
收录类别	ESCI ; SCIE
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
WOS类目	Biochemistry & Molecular Biology ; Plant Sciences
WOS记录号	WOS:000718126200001
出版者	ELSEVIER
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/131982
专题	生命科学与技术学院_博士生生命科学与技术学院_特聘教授组_龚继明组
通讯作者	Gong, Ji-Ming
作者单位	1.Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, Shanghai Inst Plant Physiol & Ecol, Natl Key Lab Plant Mol Genet, Shanghai 200032, Peoples R China; 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China; 4.Henan Univ, Sch Life Sci, State Key Lab Crop Stress Adaptat & Improvement, Kaifeng 475004, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Chen, Si-Ying,Gu, Tian-Yu,Qi, Zi-Ai,et al. Two NPF transporters mediate iron long-distance transport and homeostasis in Arabidopsis[J]. PLANT COMMUNICATIONS,2021,2(6).
APA	Chen, Si-Ying.,Gu, Tian-Yu.,Qi, Zi-Ai.,Yan, Jing.,Fang, Zi-Jun.,...&Gong, Ji-Ming.(2021).Two NPF transporters mediate iron long-distance transport and homeostasis in Arabidopsis.PLANT COMMUNICATIONS,2(6).
MLA	Chen, Si-Ying,et al."Two NPF transporters mediate iron long-distance transport and homeostasis in Arabidopsis".PLANT COMMUNICATIONS 2.6(2021).