Egr1-EGFP transgenic mouse allows in vivo recording of Egr1 expression and neural activity

doi:10.1016/j.jneumeth.2021.109350

	Egr1-EGFP transgenic mouse allows in vivo recording of Egr1 expression and neural activity
	Wang, Guangyu 1,2; Xie, Hong 3; Hu, Yi 1; Chen, Qinan1 ; Liu, Chenhui1 ; Liu, Kaiyuan 4; Yan, Yuze 4; Guan, Ji-Song1
	2021-11-01
发表期刊	JOURNAL OF NEUROSCIENCE METHODS (IF:2.7[JCR-2023],2.7[5-Year])
ISSN	0165-0270
EISSN	1872-678X
卷号	363
DOI	10.1016/j.jneumeth.2021.109350
摘要	Background: Immediate-early genes (IEGs) have been serving as markers of active neurons for their rapid responses to stimulation. With the development of IEG-EGFP reporters by the GENSAT project, application of the IEGs have been greatly expanded. However, detailed validations for these systems are still lacking, causing trouble in the interpretation of the fluorescence signals. New method: In this work, taken Egr1-EGFP transgenic mice as an example, we proposed an improvement for the usage of the Egr1-EGFP reporter system based on detailed validation of its fluorescence signals. Results: Firstly, the exogenous EGFP mRNA levels were linearly correlated with the endogenous Egr1 mRNA levels in neurons. Secondly, the 3-hr-changes of the Egr1-EGFP signals before and after the stimulus were positively correlated with the stimulus-induced neuronal activities. Interestingly, persistent neuronal activity patterns in the post-stimulus phase also showed correlation with the stimulus-induced Egr1-EGFP signal changes. Furthermore, enriched environments engaged dramatic neuronal activations, allowing detailed characterization of Egr1EGFP expression dynamics Comparison with existing method(s): People used to infer the neuronal activities based on the raw fluorescence signals of IEG-EGFP reporter system, which was strongly obstructed by distinct protein regulation or dynamic properties between the EGFP and the IEGs. We demonstrated a better way for data analysis and experimental design. Conclusions: Taken together, this work proves that Egr1-EGFP signal is weakly but significantly correlated to task induced neural activity and gives detailed characterization of the signal dynamics. It not only provides basis for the understanding of the IEG-EGFP fluorescence signals but also offers instructions for proper experimental design with IEG-EGFP reporter systems.
关键词	Immediate-early gene Egr1-EGFP transgenic mouse in vivo imaging Activity-dependent Enriched environment
收录类别	SCIE
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Neurosciences & Neurology
WOS类目	Biochemical Research Methods ; Neurosciences
WOS记录号	WOS:000704348900008
出版者	ELSEVIER
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/128413
专题	生命科学与技术学院_博士生生命科学与技术学院_PI研究组_管吉松组
通讯作者	Guan, Ji-Song
作者单位	1.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China; 2.Inst Brain Intelligence Sci & Technol, Shanghai Res Ctr Brain Sci & Brain Inspired Intel, Shanghai 201210, Peoples R China; 3.Univ Shanghai Sci & Technol, Ctr Artificial Intelligence Nanophoton, Shanghai 200093, Peoples R China; 4.Tsinghua Univ, Sch Life Sci, IDG McGovern Inst Brain Res Tsinghua, MOE Key Lab Prot Sci, Beijing 100084, Peoples R China
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
第一作者的第一单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Wang, Guangyu,Xie, Hong,Hu, Yi,et al. Egr1-EGFP transgenic mouse allows in vivo recording of Egr1 expression and neural activity[J]. JOURNAL OF NEUROSCIENCE METHODS,2021,363.
APA	Wang, Guangyu.,Xie, Hong.,Hu, Yi.,Chen, Qinan.,Liu, Chenhui.,...&Guan, Ji-Song.(2021).Egr1-EGFP transgenic mouse allows in vivo recording of Egr1 expression and neural activity.JOURNAL OF NEUROSCIENCE METHODS,363.
MLA	Wang, Guangyu,et al."Egr1-EGFP transgenic mouse allows in vivo recording of Egr1 expression and neural activity".JOURNAL OF NEUROSCIENCE METHODS 363(2021).